Efficient electrospray ionization from polymer microchannels using integrated hydrophobic membranes.
Efficient electrospray ionization from polymer microchannels using integrated hydrophobic membranes.
复制标题
使用集成疏水膜从聚合物微通道进行高效电喷雾电离。
DOI：
10.1039/b402825b
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发表时间：
2004
期刊：
影响因子：
6.1
通讯作者：
DeVoe,DonL
中科院分区：
文献类型：
--
作者：
Wang,Ying-Xin;Cooper,JonW;Lee,ChengS;DeVoe,DonL
描述了一种在聚合物微流控系统中实现稳定可靠的电喷雾离子化（ESI）尖端的简单方法。该过程基于在微通道出口处添加薄疏水性膜，以限制ESI期间形成的泰勒锥的横向分散。使用这种方法，通过光学成像，ESI芯片在低至80 nL min−1的流速下显示出定义良好的泰勒锥。此外，在几个小时的连续操作中，已经测量到低至10 nL min−1的流量下的稳定电喷雾电流。表征电喷雾过程中制造的ESI芯片的光学和电学监测的报告，连同使用肌红蛋白作为模型蛋白质的质谱验证。该新方法提供了低成本的潜力，一次性聚合物微流体分离平台的直接接口到质谱。
A simple process for realizing stable and reliable electrospray ionization (ESI) tips in polymer microfluidic systems is described. The process is based on the addition of a thin hydrophobic membrane at the microchannel exit to constrain lateral dispersion of the Taylor cone formed during ESI. Using this approach, ESI chips are shown to exhibit well-defined Taylor cones at flow rates as low as 80 nL min−1 through optical imaging. Furthermore, stable electrospray current has been measured for flow rates as low as 10 nL min−1 over several hours of continuous operation. Characterization of the electrospray process by optical and electrical monitoring of fabricated ESI chips is reported, together with mass spectrometry validation using myoglobin as a model protein. The novel process offers the potential for low-cost, direct interfacing of disposable polymer microfluidic separation platforms to mass spectrometry.