Preparation of multilayer thin films containing avidin through sugar-lectin interactions and their binding properties
Preparation of multilayer thin films containing avidin through sugar-lectin interactions and their binding properties
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DOI：
10.1021/la0203685
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发表时间：
2002-09-03
期刊：
影响因子：
3.9
通讯作者：
Anzai, J
中科院分区：
文献类型：
--
作者：
Hoshi, T;Akase, S;Anzai, J
通过伴刀豆球蛋白A（Con A）与连接在抗生物素蛋白分子表面的糖链之间的强生物亲和力，在石英玻片表面逐层沉积抗生物素蛋白和伴刀豆球蛋白A，成功制备了含抗生物素蛋白的蛋白质薄膜。紫外 - 可见吸收光谱和石英晶体微天平研究表明，抗生物素蛋白在多层膜的每一层中几乎形成单分子层。构建在多层膜中的抗生物素蛋白分子保留了其与生物素和类似物2 - （（4'-羟基苯基）偶氮）苯甲酸的结合活性。抗生物素蛋白 - Con A薄膜紧密结合生物素标记的染料（荧光素）。抗生物素蛋白 - Con A多层膜在缓冲溶液或低浓度葡萄糖和甘露糖溶液（10 mM）中是稳定的。
Protein thin films containing avidin were successfully prepared by a layer-by-layer deposition of avidin and concanavalin A (Con A) on the surface of a quartz slide, through a strong bioaffinity between Con A and sugar chains attached to the surface of avidin molecule. The UV-visible absorption spectroscopy and quartz-crystal microbalance studies revealed that avidin forms nearly a monomolecular layer in each layer of the multilayer films. Avidin molecules built into the multilayer films retained their binding activity to biotin and analogue 2-((4'-hydroxyphenyl)azo)benzoic acid. The avidin-Con A films bound biotin-tagged dye (fluorescein) tightly. The avidin-Con A multilayer films are stable in buffer solution or in low concentration of glucose and mannose solutions (10 mM).