A MODIFIED AGAR PLATE METHOD FOR DETECTION OF STRONGYLOIDES-STERCORALIS
A MODIFIED AGAR PLATE METHOD FOR DETECTION OF STRONGYLOIDES-STERCORALIS
复制标题
DOI：
10.4269/ajtmh.1991.45.518
复制
发表时间：
1991-10-01
影响因子：
3.3
通讯作者：
OHTOMO, H
中科院分区：
文献类型：
--
作者：
KOGA, K;KASUYA, S;OHTOMO, H
琼脂平板法是一种新的诊断人体弓形虫病的新技术，具有较高的检出率。这份报告详细介绍了对该技术的修改，并建立了一个标准化的程序。我们建议所有的平板都应该用显微镜仔细观察，因为宏观观察可能导致假阴性结果。还建议将福尔马林溶液直接倒入镜检阳性的培养皿中，通过沉淀收集蠕虫。这一过程使人们能够观察到隐藏的蠕虫。用胶带密封培养皿，防止幼虫爬出培养皿，消除了任何降低检测率的可能性，极大地改善了技术人员执行该程序的安全条件。我们认为琼脂平板法在检测类圆线虫方面优于滤纸法，我们相信它最终会成为首选的技术。
The agar plate method is a new technique with high detection rates for coprological diagnosis of human strongyloidiasis. This report details modifications of the technique and establishes a standardized procedure. We recommend that all plates should be carefully observed using a microscope because macroscopic observation can lead to false negative results. It is also advisable to pour formalin solution directly into microscopically positive dishes to collect worms by sedimentation. This procedure enables one to observe worms otherwise hidden. Sealing dishes with adhesive tape prevents larvae from crawling out of the dishes, eliminating any possibility in the reduction of detection rates, and greatly improves the safety conditions for the technician performing the procedure. We consider the agar plate method to be superior to the filter paper method in detecting Strongyloides, and we believe that it will eventually become the technique of choice.