Characterization of the link protein from bovine epiphyseal plate.
Characterization of the link protein from bovine epiphyseal plate.
复制标题
牛骨骺板连接蛋白的表征。
DOI：
10.1016/s0006-291x(80)80094-5
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发表时间：
1980
影响因子：
3.1
通讯作者：
Mankin,HJ
中科院分区：
文献类型：
--
作者：
Amadio,PC;Treadwell,BV;Towle,CA;Mankin,HJ
用本实验室介绍的一种方法分离了牛骨板软骨的连接蛋白组份。氨基酸分析、聚丙烯酰胺凝胶电泳和玻璃体蛋白多糖聚集研究得出的结果与先前报道的牛鼻和关节连接的结果相似。对骨骺板和关节连接的免疫扩散研究表明，这两个组织具有免疫上无法区分的连接部分。与在其他牛软骨中一样，发现了两个主要的连接蛋白，但较低分子量的蛋白在骨痂中比在鼻或关节软骨中更突出。
The link protein fraction of bovine epiphyseal plate cartilage was isolated using a method previously described by this laboratory. Amino acid analysis, polyacrylamide gel electrophoresis, andin vitroproteoglycan aggregation studies gave results similar to those previously reported for bovine nasal and articular links. Immunodiffusion studies of epiphyseal plate and articular links showed that the two tissues have immunologically indistinguishable link fractions. As in other bovine cartilages, two main link proteins were identified, but the lower molecular weight protein was more prominent in epiphyseal than in nasal or articular cartilage.