Analysis of force curves obtained on the live cell membrane using chemically modified AFM probes
Analysis of force curves obtained on the live cell membrane using chemically modified AFM probes
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DOI：
10.1016/j.ultramic.2004.01.013
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发表时间：
2004-08-01
期刊：
影响因子：
2.2
通讯作者：
Ikai, A
中科院分区：
文献类型：
--
作者：
Afrin, R;Yamada, T;Ikai, A
力曲线上获得的活细胞表面上使用原子力显微镜安装与双功能共价交联剂，二琥珀酰亚胺辛二酸酯，形成一个共价键与氨基轴承分子的细胞表面上的修改后的尖端。在力曲线的收缩状态开始之前引入1.0 s的斜坡延迟时间，以增加尖端上的交联剂与细胞表面上的氨基之间的静止反应时间。虽然活细胞表面的反应，强制接触的非功能化的尖端很少表现出证据的尖端-细胞相互作用，那些得到了修改后的提示给出了明确的指示，延长的粘附，这是终止了一个单一的步骤释放的尖端到其中性位置。在本工作给定的实验条件下，在总共198条力曲线中，58%的力曲线只给出一个跳跃，而有一个跳跃的力曲线中，70%的力曲线给出的最终断裂力为4.5+/-0.22nN。结果强调了用化学修饰的尖端探测时所观察到的细胞表面的机械响应的独特性。(C)2004 Elsevier B. V.保留所有权利。
Force curves were obtained on the live cell surface using an atomic force microscope mounted with a modified tip with the bifunctional covalent crosslinker, disuccinimidyl suberate, which forms a covalent bond with amino-bearing molecules on the cell surface. A ramp delay time of 1.0 s was introduced before the start of the retraction regime of the force curve to increase the stationary reaction time between the crosslinkers on the tip and the amino groups on the cell surface. While live cell surface responses to forced contact with a non-functionalized tip rarely showed evidence of tip-cell interaction, those obtained with modified tips gave clear indication of prolonged adhesion which was terminated by a single step release of the tip to its neutral position. Under the given experimental conditions of this work, 58% of a total of 198 force curves gave only one jump and 70% of those with one jump gave the final rupture force of 4.5+/-0.22nN. The result emphasized the uniqueness of the observed mechanical response of the cell surface when probed with chemically modified tips. (C) 2004 Elsevier B.V. All rights reserved.