Effects of fish protein with glycation extent on gut microbiota and colonic barrier function in mice fed a high-fat diet
Effects of fish protein with glycation extent on gut microbiota and colonic barrier function in mice fed a high-fat diet
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具有糖化程度的鱼蛋白对高脂饮食小鼠肠道微生物群和结肠屏障功能的影响
DOI：
10.1016/j.jff.2021.104636
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发表时间：
2021-07-27
影响因子：
5.6
通讯作者：
Wu, Haohao
中科院分区：
文献类型：
--
作者：
Cao, Caocao;Tang, Mingjun;Wu, Haohao
本研究旨在研究具有糖化程度的鱼类蛋白如何影响高脂饮食小鼠的肠道微生物群和结肠屏障功能。给小鼠喂食鱼蛋白（MP）饲料，低、中、高糖化程度的鱼蛋白（L-GE、M-GE和H-GE）饲料15周。糖基化鱼蛋白饲料显著提高了丁酸的生成，这与丁酰辅酶A：乙酸辅酶A转移酶（BUT）和丁酸激酶（BUK）基因的表达有关;糖基化鱼蛋白饲料显著改善了胰岛素抵抗，但增加了AGEs受体（AGEs receptor，AGEs receptor，BUT）的表达。在4种日粮中，L-GE日粮显著提高了occudin和zonulin-1（ZO-1）的含量，降低了炎症指标，提高了丁酸产生菌Lachnospiraceae_UCG-006和Allobaculum的含量，而H-GE日粮降低了双歧杆菌和乳酸杆菌的含量。总的来说，这些生物学效应受鱼蛋白的糖基化程度的影响很大，并且具有低糖基化程度的鱼蛋白可能对肠道健康提供更潜在的积极作用。
This study aimed to investigate how fish protein with glycation extent affects gut microbiota and colonic barrier function in mice fed a high-fat diet. The mice were fed a fish protein (MP) diet, fish protein with low, medium and high glycation extent (L-GE, M-GE and H-GE) diet for 15 weeks. The glycated fish protein diets greatly increased butyrate production, which was related to the genes expression of butyryl-CoA: acetate CoAtransferase (BUT) and butyrate kinase (BUK), and also considerably improved insulin resistance, but increased the expression of AGEs receptor (RAGE). Among the four diets, the L-GE diet markedly increased occudin and zonulin-1 (ZO-1), lowered inflammation markers, and enhanced butyrate-producing bacteria Lachnospiraceae_UCG-006 and Allobaculum, while H-GE diet decreased Bifidobacterium and Lactobacillus. Overall, these biological effects were greatly affected by the glycation extent of fish protein, and the fish protein with low glycation extent may provide more potentially positive effects to gut health.