Expression of B7-H4 and hepatitis B virus X in hepatitis B virus-related hepatocellular carcinoma
Expression of B7-H4 and hepatitis B virus X in hepatitis B virus-related hepatocellular carcinoma
复制标题
B7-H4和乙型肝炎病毒X在乙型肝炎病毒相关性肝细胞癌中的表达
DOI：
10.3748/wjg.v22.i18.4538
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发表时间：
2016-05-14
影响因子：
4.3
通讯作者：
Yao, Hang-Ping
中科院分区：
文献类型：
--
作者：
Hong, Bo;Qian, Yun;Yao, Hang-Ping
目的：为探讨B7-H4和B肝炎病毒X（HBx）蛋白在B肝炎病毒相关性肝癌（HBV-HCC）中的表达及其临床意义，采用免疫印迹、流式细胞术和免疫荧光法检测B7-H4在人肝癌细胞系HepG 2和HepG2.2.15中的表达。采用免疫组化法检测83例HBV-HCC中B7-H4和HBx的表达，并分析其与临床病理特征的关系。石蜡切片由入组本研究的83名HBV-HCC患者（22名女性和61名男性）产生。这些患者的年龄范围为35至77岁，平均年龄为52.5 ± 11.3岁。结果：B7-H4在HepG2.2.15细胞中的表达明显高于HepG 2细胞。B7-H4蛋白在HepG 2细胞裂解液中的表达高于HepG2.2.15细胞。此外，HBx仅在HepG2.2.15细胞中表达。通过流式细胞术获得类似的数据。83例HBV-HCC组织中B7-H4和HBx的阳性率分别为68.67%（57/83）和59.04%（49/83）。HBx的表达与TNM分期有关，B7-H4的表达与HBx呈正相关（r（s）= 0.388; P < 0.01）。HBx阳性的HBV-HCC组织中B7-H4的表达水平明显高于HBx阴性的HBV-HCC组织。结论：HBx和B7-H4的高表达与HBV-HCC的肿瘤进展有关，提示B7-H4可能参与了HBV相关性肝癌的发生。
AIM: To investigate the expression and clinical significance of B7-H4 and hepatitis B virus X (HBx) protein in hepatitis B virus-related hepatocellular carcinoma (HBV-HCC).METHODS: The expression of B7-H4 in the human HCC cell lines HepG2 and HepG2.2.15 were detected by western blot, flow cytometry, and immunofluorescence. The expression of B7-H4 and HBx in 83 HBV-HCC was detected by immunohistochemistry, and the relationship with clinicopathological features was analyzed. Paraffin sections were generated from 83 HBV-HCC patients (22 females and 61 males) enrolled in this study. The age of these patients ranged from 35 to 77 years, with an average of 52.5 +/- 11.3 years. All experiments were approved by the Ethics Committees of the Second Affiliated Hospital, Zhejiang University School of Medicine.RESULTS: B7-H4 was significantly upregulated in HepG2.2.15 cells compared to HepG2 cells. Specifically, the protein expression of B7-H4 in the lysates of HepG2 cells was more than that in HepG2.2.15 cells. In addition, HBx was expressed only in HepG2.2.15 cells. Similar data were obtained by flow cytometry. The positive rates of B7-H4 and HBx in the tissues of 83 HBV-HCC patients were 68.67% (57/83) and 59.04% (49/83), respectively. The expression of HBx was correlated with tumor node metastases (TNM) stage, and the expression of B7-H4 was positively correlated with HBx (r(s) = 0.388; P < 0.01). The expression level of B7-H4 in HBx-positive HBV-HCC tissues was substantially higher than that in HBx-negative HBV-HCC tissues. The expression level of B7H4 was negatively related to tumor TNM stage.CONCLUSION: Higher expression of HBx and B7-H4 was correlated with tumor progression of HBV-HCC, suggesting that B7-H4 may be involved in facilitating HBV-related hepatocarcinogenesis.