Powdery mildew disease resistance and marker-assisted screening at the Pm60 locus in wild diploid wheat Triticum urartu
Powdery mildew disease resistance and marker-assisted screening at the Pm60 locus in wild diploid wheat Triticum urartu
复制标题
野生二倍体小麦乌拉尔图白粉病抗性及Pm60基因座标记辅助筛选
DOI：
10.1016/j.cj.2019.09.007
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发表时间：
2020-04-01
期刊：
影响因子：
6.6
通讯作者：
Shen, Qian-Hua
中科院分区：
文献类型：
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作者：
Zhao, Fukai;Li, Yinghui;Shen, Qian-Hua
乌拉尔小麦（Triticum urartu）是一种二倍体野生小麦，是小麦A基因组的祖先，是抗白粉病的重要资源。（Bgt.）在本研究中，我们系统地表征了Bgt真菌和T.在微观层面上。我们还测试了227 T。urartu种质对Bgt分离物E09的反应，并在该收集物中发现了先前未分类的白粉病抗性。Pm 60是具有至少三个功能等位基因Pm 60、Pm 60 a和Pm 60 b的CC-NB-LRR型白粉病抗性基因。靶向Pm 60基因座的标记辅助筛选鉴定了Pm 60 a的非功能性等位基因，命名为Pm 60 a '。Pm 60 a '和Pm 60 a的序列比较显示，它们存在58个SNP和一个3-核苷酸缺失。基于序列变异，开发了两个分子标记来区分功能性Pm 60 a等位基因与非功能性Pm 60 a '。我们的筛选揭示了一个以前未表征的白粉病抗性T。urartu，并提供了新的见解Pm 60基因座。我们认为，这两个分子标记和新的T。urartu抗性材料将有助于进一步鉴定新的白粉病抗性基因，并有利于白粉病抗性育种。(c)2019中国农业科学院作物科学研究所中国作物学会Elsevier B. V.代表KeAi Communications Co.制作和主办，这是CC BY-NC-ND许可下的开放获取文章（http：//creativecommons.org/licenses/by-nc-nd/4.0/）
Triticum urartu, a diploid wild wheat and progenitor of the A genome of bread wheat, is an important resource for resistance to powdery mildew fungus caused by Blumeria graminis f. sp. tritici (Bgt). In the present study we systematically characterized the interaction between the Bgt fungus and T. urartu at the microscopic level. We also tested 227 T. urartu accessions for reaction to Bgt isolate E09 and discovered previously uncategorized powdery mildew resistance in this collection. Pm60 is a CC-NB-LRR type powdery mildew resistance gene that has at least three functional alleles, Pm60, Pm60a, and Pm60b. A marker-assisted screen targeting the Pm60 locus identified a non-functional allele of Pm60a, designated as Pm60a'. A sequence comparison of Pm60a' and Pm60a revealed that they differed by 58 SNPs and one 3-nucleotide deletion. Based on the sequence variations two molecular markers were developed to differentiate the functional Pm60a allele from the non-functional Pm60a'. Our screen revealed the presence of a previously uncharacterized powdery mildew resistance in T. urartu and provides new insights into the Pm60 locus. We believe that the two molecular markers developed here and new T. urartu resistant accessions will facilitate further identification of novel powdery mildew resistance genes and benefit breeding for powdery mildew resistance. (c) 2019 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)