Correlation between PTEN Expression and PI3K/Akt Signal Pathway in Endometrial Carcinoma
Correlation between PTEN Expression and PI3K/Akt Signal Pathway in Endometrial Carcinoma
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子宫内膜癌中PTEN表达与PI3K/Akt信号通路的相关性
DOI：
10.1007/s11596-009-0112-6
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发表时间：
2009-02-01
影响因子：
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通讯作者：
Ma, Ding
中科院分区：
文献类型：
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作者：
Gao, Qinglei;Ye, Fei;Ma, Ding
为了探讨PTEN表达在子宫内膜癌发生、发展中的作用，明确PTEN、PI3K/Akt通路与子宫内膜癌是否及如何相关，采用半定量RT-PCR方法和Western-blot方法检测24例子宫内膜癌、10例子宫内膜不典型增生、10例子宫内膜癌中PTEN和磷酸化Akt的表达情况。增生及正常子宫内膜10例。采用SP免疫组化方法检测增殖期子宫内膜31例、分泌期子宫内膜30例、增生期子宫内膜71例、不典型增生25例、子宫内膜癌73例5个研究组的PTEN蛋白表达水平。增殖期PTEN免疫染色评分为3.39±0.15，分泌期为1.90±0.21，子宫内膜增生为3.34±0.29，不典型增生为0.62±0.11，子宫内膜癌为0.74±0.19。正常子宫内膜、子宫内膜增生、子宫内膜不典型增生和子宫内膜癌中PTEN mRNA相对值分别为2.45±0.51、2.32±0.32、0.46±0.11和0.35±0.13。子宫内膜癌和不典型增生患者中PTEN mRNA和蛋白的表达水平显着低于增殖期和子宫内膜增生患者。子宫内膜癌患者中PTEN表达水平与组织类型显着相关(P
In order to investigate the role of the PTEN expression in carcinogenesis and development of endometrial carcinoma and clarify whether and how PTEN and PI3K/Akt pathway relate to endometrial carcinoma, the expression of PTEN and phospho-Akt was detected by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) methods and Western-blot from 24 cases of endometrial carcinoma, 10 cases of endometrial atypical hyperplasia, 10 cases of endometrial hyperplasia, and 10 cases of normal endometrium. SP immunohistochemical methods were used to measure levels of PTEN protein expression in following 5 study groups: 31 cases of endometrium in proliferative phase, 30 cases of endometrium in secretory phase, 71 cases of endometrial hyperplasia, 25 cases of atypical hyperplasia and 73 cases of endometrial carcinoma. Immunostaining score of PTEN was 3.39 +/- 0.15 in proliferative phase, 1.90 +/- 0.21 in secretory phase, 3.34 +/- 0.29 in endometrial hyperplasia, 0.62 +/- 0.11 in atypical hyperplasia, and 0.74 +/- 0.19 in endometrial carcinoma, respectively. PTEN mRNA relative value in normal endometrium, endometrial hyperplasia, endometrial atypical hyperplasia, and endometrial carcinoma was 2.45 +/- 0.51, 2.32 +/- 0.32, 0.46 +/- 0.11, and 0.35 +/- 0.13 respectively. The expression levels of PTEN mRNA and protein in patients with endometrial carcinoma and atypical hyperplasia were significantly lower than in those of proliferative phase and with endometrial hyperplasia. The level of PTEN expression in patients with endometrial carcinoma was significantly related to tissue type (P