A comparison of mutations induced by accelerated iron particles versus those induced by low earth orbit space radiation in the FEM-3 gene of Caenorhabditis elegans
A comparison of mutations induced by accelerated iron particles versus those induced by low earth orbit space radiation in the FEM-3 gene of Caenorhabditis elegans
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DOI：
10.1016/s0027-5107(00)00154-8
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发表时间：
2001-03-01
影响因子：
2.3
通讯作者：
Nelson, GA
中科院分区：
文献类型：
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作者：
Hartman, PS;Hlavacek, A;Nelson, GA
利用秀丽隐杆线虫fem-3基因研究了STS-76航天飞机低轨道空间辐射诱发线虫fem-3基因突变的频率和性质。将这些突变与由布鲁克海文国家实验室的AGS同步加速器产生的加速铁离子诱导的突变进行比较。由于后勤原因，道尔幼虫在TCU准备，运送到肯尼迪航天中心或布鲁克海文国家实验室，在太空中飞行或辐照，返回TCU并筛选突变体。在穿梭飞行后，总共回收了25个fem-3突变体，突变频率为2.1 × 10 ~（-5）。大约是自发速率6.3 × 10 - 6的3.3倍。其中四个突变是纯合的，这表明它们是包含fem-3以及邻近的必需基因的大缺失。Southern印迹分析显示，25个中有一个在fem-3中含有多态性，这进一步证明了空间辐射可以诱导缺失。虽然没有多态性之间检测到铁离子诱导的突变，15个突变体中的3个是纯合的不可行的。这与先前观察到的高LET铁颗粒产生缺陷一致。这些数据提供了证据，尽管是间接的，环境空间辐射的一个重要诱变成分是高LET带电粒子，如铁离子。(C)2001 Elsevier Science B. V.保留所有权利。
The fem-3 gene of Caenorhabditis elegans was employed to determine the mutation frequency as well as the nature of mutations induced by low earth orbit space radiation ambient to Space Shuttle flight STS-76. Recovered mutations were compared to those induced by accelerated iron ions generated by the AGS synchrotron accelerator at Brookhaven National Laboratory. For logistical reasons, dauer larvae were prepared at TCU, transported to either Kennedy Space Center or Brookhaven National Laboratory, flown in space or irradiated, returned to TCU and screened for mutants. A total of 25 fem-3 mutants were recovered after the shuttle flight and yielded a mutation frequency of 2.1 x 10(-5). roughly 3.3-fold higher than the spontaneous rate of 6.3 x 10(-6). Four of the mutations were homozygous inviable, suggesting that they were large deletions encompassing fem-3 as well as neighboring, essential genes. Southern blot analyses revealed that one of the 25 contained a polymorphism in fem-3, further evidence that space radiation can induce deletions. While no polymorphisms were detected among the iron ion-induced mutations, three of the 15 mutants were homozygous inviable. which is in keeping with previous observations that high LET iron particles generate deficiencies. These data provide evidence, albeit indirect, that an important mutagenic component of ambient space radiation is high LET charged particles such as iron ions. (C) 2001 Elsevier Science B.V. All rights reserved.