Transcutaneous lactate monitoring based on a micro-planar amperometric biosensor
Transcutaneous lactate monitoring based on a micro-planar amperometric biosensor
复制标题
基于微平面电流生物传感器的经皮乳酸监测
DOI：
10.1016/0003-2670(95)00216-m
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
I. Karube
中科院分区：
文献类型：
--
作者：
N. Ito;T. Matsumoto;H. Fujiwara;Y. Matsumoto;S. Kayashima;T. Arai;M. Kikuchi;I. Karube
将吸液采集技术与微平面电流型生物传感器相结合，发展了一种经皮乳酸监测方法。通过去除角质层的皮肤用微弱的吸力直接收集自来水。还开发了一种微型平面生物传感器，用于测定非常小的样品中的乳酸浓度。该生物传感器利用微平面铂电极上的乳酸氧化酶固定膜制成乳酸传感器。该传感器基于电流法检测乳酸氧化酶催化的L-乳酸氧化生成的过氧化氢。该传感器在0.5~25 mm范围内具有良好的线性关系，响应时间约为10 S，并将该传感器应用于兔诱导高乳酸状态下的场效应态乳酸测定。SEF测定的乳酸浓度与直接从全血中测定的乳酸浓度具有良好的相关性。这一结果表明，经皮乳酸监测是可行的，不需要采血。
A transcutaneous lactate monitoring method has been developed by combining a suction effusion fluid (SEF) collecting technique with a micro-planar amperometric biosensor. SEF is directly collected by a weak suction through the skin from which the stratum corneum has been removed. A micro-planar biosensor, has also been developed for determining lactate concentrations in very small samples. The biosensor utilizes a lactate oxidase immobilized membrane on a micro planar platinum electrode to yield a lactate sensor. The sensor is based on amperometric detection of hydrogen peroxide generated by the lactate oxidase-catalyzed oxidation of l-lactate. The linearity of the lactate sensor response was obtained from 0.5 mM to 25 mM and the response time was about 10 s. The sensor was applied to the SEF lactate measurement of a rabbit during induced high lactate levels. Lactate concentrations measured with SEF showed good correlation with those obtained directly from whole blood. This result suggests the feasibility of transcutaneous lactate monitoring without the use of blood sampling.