Validated MALDI-TOF/TOF mass spectra for protein standards
Validated MALDI-TOF/TOF mass spectra for protein standards
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DOI：
10.1016/j.jasms.2007.01.010
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发表时间：
2007-05-01
影响因子：
3.2
通讯作者：
Andrews, Philip C.
中科院分区：
文献类型：
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作者：
Falkner, Jayson A.;Kachman, Maureen;Andrews, Philip C.
蛋白质组学研究的当前焦点是在未知样品中蛋白质鉴定的分配中建立可接受的置信度。新算法方法的开发将极大地受益于用于测试和训练目的的已知蛋白质的标准参考光谱集。在这里，我们描述了一个开放的图书馆的ABI 4700 MALDI TOF/TOF从246个已知的，单独纯化和胰蛋白酶消化的蛋白质样品产生的质谱。Aurum数据集的初始完整版本包括凝胶图像、峰列表、光谱、搜索结果文件、诱饵数据库分析文件、蛋白质序列的FASTA文件、手动管理和描述蛋白质覆盖率和通过MS/MS匹配的肽的摘要页面，然后使用Mascot、Sequest和X！串联这些数据可在ProteomeCommons.org上公开使用。
A current focus of proteomics research is the establishment of acceptable confidence measures in the assignment of protein identifications in an unknown sample. Development of new algorithmic approaches would greatly benefit from a standard reference set of spectra for known proteins for the purpose of testing and training. Here we describe an openly available library of mass spectra generated on an ABI 4700 MALDI TOF/TOF from 246 known, individually purified and trypsin-digested protein samples. The initial full release of the Aurum Dataset includes gel images, peak lists, spectra, search result files, decoy database analysis files, FASTA file of protein sequences, manual curation, and summary pages describing protein coverage and peptides matched by MS/MS followed by decoy database analysis using Mascot, Sequest, and X!Tandem. The data are publicly available for use at ProteomeCommons.org.