Genetic analysis of seed-soluble oligosaccharides in relation to seed storability of Arabidopsis
Genetic analysis of seed-soluble oligosaccharides in relation to seed storability of Arabidopsis
复制标题
DOI：
10.1104/pp.124.4.1595
复制
发表时间：
2000-12-01
期刊：
影响因子：
7.4
通讯作者：
Koornneef, M
中科院分区：
文献类型：
--
作者：
Bentsink, L;Alonso-Blanco, C;Koornneef, M
种子低聚糖（OS），特别是棉子糖系列OS（RSOs）被认为在获得脱水耐受性，从而在种子耐贮藏性中起重要作用。在目前的工作中，我们分析了种子可溶性OS（蔗糖，棉子糖，水苏糖）含量的几个拟南芥加入，从而确定了基因型佛得角群岛具有非常低的RSO含量。通过对重组自交系群体进行数量性状基因座（QTL）定位，我们发现了一个主要的QTL负责种子水苏糖含量的实际单基因分离。该位点也影响了另外两种OS、蔗糖和棉子糖的含量。两个候选基因分别编码半乳糖醇合酶和棉子糖合酶位于该主效QTL的基因组区域内。此外，三个较小的影响QTL被确定，每一个具体影响个人OS的内容。种子耐贮藏性进行了分析，在相同的重组自交系人口通过测量两个不同的种子老化试验的活力（发芽）：自然老化后，在室温下干燥储存4年，人工老化后，由受控变质试验诱导。因此，4个QTL负责该性状的变化进行了定位。QTL遗传位置的比较表明，基因组中含有主效OS位点的区域对种子耐贮性没有显著影响。我们的结论是，在所研究的材料中，RSO和蔗糖含量对种子耐贮性都没有特定的影响。
Seed oligosaccharides (OSs) and especially raffinose series OSs (RSOs) are hypothesized to play an important role in the acquisition of desiccation tolerance and consequently in seed storability. In the present work we analyzed the seed-soluble OS (sucrose, raffinose, and stachyose) content of several Arabidopsis accessions and thus identified the genotype Cape Verde Islands having a very low RSO content. By performing quantitative trait loci (QTL) mapping in a recombinant inbred line population, we found one major QTL responsible for the practically monogenic segregation of seed stachyose content. This locus also affected the content of the two other OSs, sucrose, and raffinose. Two candidate genes encoding respectively for galactinol synthase and raffinose synthase were located within the genomic region around this major QTL. In addition, three smaller-effect QTL were identified, each one specifically affecting the content of an individual OS. Seed storability was analyzed in the same recombinant inbred line population by measuring viability (germination) under two different seed aging assays: after natural aging during 4 years of dry storage at room temperature and after artificial aging induced by a controlled deterioration test. Thus, four QTL responsible for the variation of this trait were mapped. Comparison of the QTL genetic positions showed that the genomic region containing the major OS locus did not significantly affect the seed storability. We concluded that in the studied material neither RSOs nor sucrose content had a specific effect on seed storability.