Sulfated modification of the polysaccharide from Sphallerocarpus gracilis and its antioxidant activities.
Sulfated modification of the polysaccharide from Sphallerocarpus gracilis and its antioxidant activities.
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DOI：
10.1016/j.ijbiomac.2016.02.037
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发表时间：
2016-06
影响因子：
8.2
通讯作者：
Yunfei Xu;Shen Song;Yanxia Wei;Fengxia Wang;Min Zhao;Jie Guo;Ji Zhang
中科院分区：
文献类型：
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作者：
Yunfei Xu;Shen Song;Yanxia Wei;Fengxia Wang;Min Zhao;Jie Guo;Ji Zhang
Sphallerocarpus gracilis(S. gracilis) is a little-investigated edible plant and used as traditional Chinese medicine. In this study, polysaccharide extracted fromS. gracilis,deproteined and purified. The polysaccharide (SGP) was chemically modified to obtain its sulfated derivatives (S-SGP) using the method of chlorosulfonic acid/pyridine (CSA/Pyr). In order to acquire the derivative with the highest degree of substitution (DS), the optimum conditions of the sulfation were obtained based on response surface design (RSD), and the structural characterizations and antioxidant properties of the S-SGP were comparatively investigated by Fourier transform infrared spectrometry (FT-IR), GC–MS analysis, size exclusion chromatography (SEC), and DPPH radical assay, hydroxyl radical assay, superoxide radical assay, and reducing power assay, respectively. Results showed that the modification was successful, and obtained the optimum combination of conditions. Compared with SGP, the sulfated polysaccharide with relatively the decreased degree of molecular weight (Mw) but the same composition of monosaccharides exhibited better antioxidant activities in DPPH, hydroxyl, superoxide radical and reducing power assay. These results indicated that the antioxidant activities in vitro of the S-SGP fromS. gracilismay be related to combined effects of Mw, monosaccharide composition, and sulfate content.
