High-level bacterial expression of mitochondrial transport proteins
High-level bacterial expression of mitochondrial transport proteins
复制标题
线粒体转运蛋白的高水平细菌表达
DOI：
--
复制
发表时间：
1996
影响因子：
3
通讯作者：
R. S. Kaplan
中科院分区：
文献类型：
--
作者：
R. S. Kaplan
The inability to obtain abundant quantities of purified, functional membrane proteins has represented a significant impediment to the goal of obtaining high-resolution structural information. In this review, procedures are described which have been developed in this laboratory and enable the high-level bacterial expression and subsequent purification of functional mitochondrial citrate transport proteins from yeast and rat liver. The data that we have obtained using these procedures and related results from other laboratories are discussed. Additionally, the general applicability of this approach to most mitochondrial transport proteins as well as to other types of membrane proteins is considered. Finally, relevant considerations when contemplating the use of this methodology and the likely value of this approach in future areas of research are explored.
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DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Sandor,A;Johnson,JH;Srere,PA
通讯作者：
Srere,PA
DOI：
10.1016/s0021-9258(19)38701-0
发表时间：
1993-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. S. Kaplan;J. Mayor;D. Wood
通讯作者：
R. S. Kaplan;J. Mayor;D. Wood
大鼠肝线粒体重建活性磷酸盐转运蛋白的纯化和表征。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kaplan,RS;Pratt,RD;Pedersen,PL
通讯作者：
Pedersen,PL
在大肠杆菌中表达的酵母线粒体磷酸转运蛋白。
影响因子：
2.9
作者：
Wohlrab,H;Briggs,C
通讯作者：
Briggs,C
DOI：
10.1073/pnas.88.13.5597
发表时间：
1991-07-01
影响因子：
11.1
作者：
BARNES, HJ;ARLOTTO, MP;WATERMAN, MR
通讯作者：
WATERMAN, MR