Interaction of Salmonella enterica serovar Typhi with cultured epithelial cells: roles of surface structures in adhesion and invasion.
Interaction of Salmonella enterica serovar Typhi with cultured epithelial cells: roles of surface structures in adhesion and invasion.
复制标题
DOI：
10.1099/mic.0.2008/016998-0
复制
发表时间：
2008-07
期刊：
影响因子：
--
通讯作者：
Dougan G
中科院分区：
文献类型：
--
作者：
Bishop A;House D;Perkins T;Baker S;Kingsley RA;Dougan G
在这项研究中，我们研究了伤寒沙门氏菌（S. Typhi）表面结构在上皮细胞检测系统中对侵袭和黏附的影响能力。总体而言，发现伤寒沙门氏菌比鼠伤寒沙门氏菌（S. Typhimurium）的黏附性、侵袭性和细胞毒性更低。培养条件对伤寒沙门氏菌与培养细胞的黏附影响较小，但对侵袭有显著影响。相比之下，细菌生长条件并不影响伤寒沙门氏菌对极化细胞的顶端侵袭。将细菌应用于极化细胞的基底外侧表面可增加伤寒沙门氏菌（而非鼠伤寒沙门氏菌）的侵袭水平。伤寒沙门氏菌毒力（Vi）荚膜的表达导致黏附适度降低，但非极化细胞的侵袭水平大幅降低。然而，Vi荚膜表达对极化细胞顶端或基底外侧表面的侵袭没有影响。staA、tcfA或pilS基因的突变在有或无Vi荚膜的情况下均不影响侵袭或黏附。
In this study we investigate the ability of Salmonella enterica serovar Typhi (S. Typhi) surface structures to influence invasion and adhesion in epithelial cell assay systems. In general, S. Typhi was found to be less adherent, invasive and cytotoxic than S. enterica serovar Typhimurium (S. Typhimurium). Culture conditions had little effect on adhesion of S. Typhi to cultured cells but had a marked influence on invasion. In contrast, bacterial growth conditions did not influence S. Typhi apical invasion of polarized cells. The levels of S. Typhi, but not S. Typhimurium, invasion were increased by application of bacteria to the basolateral surface of polarized cells. Expression of virulence (Vi) capsule by S. Typhi resulted in a modest reduction in adhesion, but profoundly reduced levels of invasion of non-polarized cells. However, Vi capsule expression had no affect on invasion of the apical or basolateral surfaces of polarized cells. Mutation of the staA, tcfA or pilS genes did not affect invasion or adhesion in either the presence or the absence of Vi capsule.
登录
查看更多内容
影响因子：
3.8
作者：
CHATFIELD, SN;STRAHAN, K;DOUGAN, G
通讯作者：
DOUGAN, G
影响因子：
3.1
作者：
Lyczak, JB;Pier, GB
通讯作者：
Pier, GB
影响因子：
3.1
作者：
PICKARD, D;LI, JL;CHATFIELD, S
通讯作者：
CHATFIELD, S
影响因子：
3.6
作者：
Arricau, N;Hermant, D;Popoff, MY
通讯作者：
Popoff, MY
影响因子：
9.4
作者：
HEESEMANN, J;LAUFS, R
通讯作者：
LAUFS, R