RESPONSE OF RAT SELENOPROTEIN-P TO SELENIUM ADMINISTRATION AND FATE OF ITS SELENIUM
RESPONSE OF RAT SELENOPROTEIN-P TO SELENIUM ADMINISTRATION AND FATE OF ITS SELENIUM
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DOI：
10.1152/ajpendo.1991.261.1.e26
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发表时间：
1991-07-01
影响因子：
--
通讯作者：
BELLEW, T
中科院分区：
文献类型：
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作者：
BURK, RF;HILL, KE;BELLEW, T
Selenoprotein P is a glycoprotein that contains > 60% of the selenium in rat plasma. Physiological experiments were undertaken to gain insight into selenoprotein P function. Selenium-deficient rats were injected with doses of selenium ranging from 25 to 200-mu-g/kg, and the appearance of selenoprotein P was compared with the appearance of glutathione peroxidase activity in plasma and in liver. Selenoprotein P concentration increased to 35% of control by 6 h, whereas glutathione peroxidase activity increased minimally or not at all. Moreover, in rats given 100 and 200-mu-g selenium/kg, selenoprotein P reached 75% of its concentration in control rats at 24 h, whereas glutathione peroxidase activity reached only 6% of control. Cycloheximide pretreatment blocked the appearance of selenoprotein P in response to selenium injection. Male and female rats had similar concentrations of selenoprotein P. Partially purified selenoprotein P and plasma glutathione peroxidase labeled with Se-75 were administered intravenously to selenium-deficient and control rats. Se-75 given as selenoprotein P disappeared more rapidly from plasma than did Se-75 given as glutathione peroxidase. Selenium deficiency did not significantly affect Se-75 disappearance from plasma. At 2 h, brain, but not other tissues, took up more Se-75 in selenium-deficient rats than in control rats when Se-75 was given as selenoprotein P. This suggests that brain has a specific uptake mechanism for selenium given in the form of selenoprotein P. These results demonstrate that several physiological properties distinguish selenoprotein P from glutathione peroxidase. However, they do not clearly indicate its function.
