Differential expression of nuclear lamin, the major component of nuclear lamina, during neurogenesis in two germinal regions of adult rat brain
Differential expression of nuclear lamin, the major component of nuclear lamina, during neurogenesis in two germinal regions of adult rat brain
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DOI：
10.1111/j.1460-9568.2007.05450.x
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发表时间：
2007-03
影响因子：
3.4
通讯作者：
Y. Takamori;Y. Tamura;Y. Kataoka;Yilong Cui;Saori Seo;Takenori Kanazawa;K. Kurokawa;H. Yamada
中科院分区：
文献类型：
--
作者：
Y. Takamori;Y. Tamura;Y. Kataoka;Yilong Cui;Saori Seo;Takenori Kanazawa;K. Kurokawa;H. Yamada
核纤层蛋白是核膜的主要结构蛋白。在哺乳动物体细胞中主要存在三种核纤层蛋白亚型A/C、B1和B2。虽然已知核纤层蛋白在几种组织中的表达水平在细胞分化期间发生变化，但对神经系统中核纤层蛋白的表达知之甚少。为了研究核纤层蛋白在成年哺乳动物脑神经元分化过程中的表达，我们对大鼠脑的两个神经源性区域：齿状回颗粒下区和侧脑室脑室下区的核纤层蛋白A/C，B1和B2进行了免疫组织化学研究。特别是，使用共聚焦显微镜分析了三种类型的细胞：GFAP阳性细胞作为初级祖细胞（干细胞），PSA-NCAM阳性细胞作为后续神经元祖细胞，以及NeuN阳性成熟神经元。在两个神经源性区域，GFAP阳性细胞具有核纤层蛋白A/C（++）、B1（++）和B2（++），PSA-NCAM阳性细胞具有核纤层蛋白A/C（-）、B1（+）和B2（++），成熟神经元具有核纤层蛋白A/C（++）、B1（+）和B2（+）。这些观察结果表明，在成年大鼠脑神经发生的特定分化阶段，表达核纤层蛋白亚型的组成是不同的。我们的研究结果表明，核板层结构的改变与神经元分化的进展。
Lamins are major structural proteins of the nuclear envelope. Three lamin subtypes, A/C, B1 and B2, predominate in mammalian somatic cells. While the expression levels of lamins in several tissues are known to change during cell differentiation, lamin expression is poorly understood in the nervous system. To investigate the expression of lamins during neuronal differentiation in the mammalian adult brain, we performed immunohistochemical studies on lamins A/C, B1 and B2 in two neurogenic regions of rat brain: the subgranular zone of the dentate gyrus and the subventricular zone of the lateral ventricle. In particular, three types of cells were analysed using confocal microscopy: GFAP‐positive cells as primary progenitor (stem) cells, PSA‐NCAM‐positive cells as subsequent neuronal progenitor cells, and NeuN‐positive mature neurons. GFAP‐positive cells possesed lamin A/C (++), B1 (++) and B2 (++), PSA‐NCAM‐positive cells possessed lamin A/C (–), B1 (+++) and B2 (+), and mature neurons possessed lamin A/C (++), B1 (+) and B2 (+++), in both neurogenic regions. These observations showed that the compositions of expressing lamin subtypes are distinct in particular differentiation stages during neurogenesis in the adult rat brain. Our results suggest that the alteration of nuclear lamina structure is coupled with the progression of neuronal differentiation.