Enhanced biostability of nanoparticle-based drug delivery systems by albumin corona
Enhanced biostability of nanoparticle-based drug delivery systems by albumin corona
复制标题
通过白蛋白电晕增强基于纳米颗粒的药物递送系统的生物稳定性。
DOI：
10.2217/nnm.14.86
复制
发表时间：
2015-01-01
期刊：
影响因子：
5.5
通讯作者：
Lin, Yun-Feng
中科院分区：
文献类型：
--
作者：
Peng, Qiang;Wei, Xue-Qin;Lin, Yun-Feng
目的：纳米粒在组织中的快速代谢限制了其长期疗效。在这项工作中，我们的目标是通过预制牛血清白蛋白(BSA)冠层来提高纳米粒子的生物稳定性。材料和方法：将聚-3-羟基丁酸酯-3-羟基己酸纳米粒与牛血清白蛋白溶液孵育形成牛血清白蛋白冠层，并用十二烷基硫酸钠-聚丙烯酰胺凝胶和X-射线光电子能谱进行表征。考察了牛血清白蛋白冠层对纳米粒药物释放、生物稳定性和生物分布的影响。结果：在牛血清白蛋白冠层存在下，纳米粒(以香豆素-6为模型药物)的释药速度明显减慢，在肝匀浆和脏器中的稳定性增强。结论：预制牛血清白蛋白冠脉可能是提高药物生物稳定性和开发长效纳米粒制剂的一种有前途的方法。
Aims: The long-term efficacy of nanoparticles is limited by their rapid metabolism in tissues. In this work, we aim to enhance nanoparticle biostability by preforming a bovine serum albumin (BSA) corona. Materials & methods: A BSA corona was formed by incubating poly-3-hydroxybutyrate-co-3-hydroxyhexanoate nanoparticles with BSA solution and confirmed by SDS-PAGE and x-ray photoelectron spectroscopy. The impacts of the BSA corona on the drug release, biostability and biodistribution of nanoparticles were investigated. Results: In the presence of the BSA corona, the drug release (coumarin-6 was used as the model drug) of nanoparticles was significantly slower and their stability in liver homogenate and in organs was enhanced. Conclusion: Preformation of a BSA corona may be a promising approach for enhancing drug biostability and for developing long-acting nanoparticle formulations.