17β-estradiol enhances neuronal differentiation of mouse embryonic stem cells
17β-estradiol enhances neuronal differentiation of mouse embryonic stem cells
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DOI：
10.1016/j.febslet.2004.05.042
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发表时间：
2004-07-02
期刊：
影响因子：
3.5
通讯作者：
Tatko, LM
中科院分区：
文献类型：
--
作者：
Murashov, AK;Pak, ES;Tatko, LM
现有的方案显示，可以从小鼠胚胎干细胞产生的神经元的百分比各不相同。在本研究中，我们比较了不同的分化条件，包括明胶和聚合L鸟氨酸/纤维连接蛋白涂层，以及神经生长因子和17-雌二醇处理对神经元总产量以及轴突生长和分支的影响。在这里，我们展示了纤维连接蛋白涂层和17-雌二醇的结合使生成的神经元数量增加了50%以上。多聚L-鸟氨酸/纤维连接蛋白可使所有培养的神经元百分率增加，提示其对神经发生有直接影响。17-雌二醇的加入减少了培养中的平均轴突长度，但显著增加了分枝。我们的结果表明，雌激素诱导ES细胞分化为神经细胞的过程中存在底物依赖的调节。(C)2004年，由Elsevier B.V.代表欧洲生化学会联合会出版。
Existing protocols show a variety in the percentage of neurons that can be generated from mouse embryonic stem (ES) cells. In the current study, we compared effects of various differentiating conditions, including gelatin and poly-L-ornithine/fibronectin coatings, and NGF and 17-estradiol treatments on the total yield of neurons, as well as, neurite growth and branching. Here, we show that combination of fibronectin coating with 17-estradiol increased number of generated neurons over 50%. Poly-L-ornithine/fibronectin increased the percent of neurons in all cultures, suggesting its direct influence on neurogenesis. Addition of 17-estradiol reduced mean neurite length in culture, but significantly increased branching. Our results indicate a substrate-dependent regulation of estrogen-induced ES cells differentiation into neuronal cells. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.