Anticancer activity of phenoxazines produced by bovine erythrocytes on colon cancer cells.
Anticancer activity of phenoxazines produced by bovine erythrocytes on colon cancer cells.
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牛红细胞产生的吩恶嗪对结肠癌细胞的抗癌活性。
DOI：
10.3892/or_00000790
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发表时间：
2010
期刊：
影响因子：
4.2
通讯作者：
A. Tomoda
中科院分区：
文献类型：
--
作者：
T. Nakachi;T. Tabuchi;A. Takasaki;S. Arai;K. Miyazawa;A. Tomoda
本研究采用改良牛红细胞悬液制备方法制备了2-氨基吩恶嗪-3-酮（Phx-3）和2-氨基-4，4 α-二氢-4 α，7-二甲基-3H-吩恶嗪-3-酮（Phx-1），并研究了它们对结肠癌细胞株COLO 201、DLD 1和PMCO 1的体外抗肿瘤活性。与使用牛血红蛋白溶液的常规方法相比，Phx-1和Phx-3的制备方法具有广泛缩短反应时间和在处理期间将样品体积减少多达七分之一的优点，从而大大减少了处理时间。由此获得的Phx-1和Phx-3通过吸收光谱和NMR光谱鉴定为纯的。这些吩恶嗪在体外对结肠癌细胞株COLO 201、DLD 1和PMCO 1具有强烈的剂量依赖性抗癌活性，并诱导这些细胞的凋亡。本研究结果表明，Phx-1和Phx-3，这是通过广泛的改进方法，使用牛红细胞制备，可能是有用的治疗药物，对结肠癌是难治性化疗。
The present study investigated the anticancer activity of 2-aminophenoxazine-3-one (Phx-3) and 2-amino-4,4 alpha-dihydro-4 alpha,7-dimethyl-3H-phenoxazine-3-one (Phx-1), which were obtained by improved preparation methods using bovine erythrocyte suspension, on colon cancer cell lines COLO201, DLD1 and PMCO1 in vitro. The preparation methods for Phx-1 and Phx-3 had the advantages of extensively shortening reaction time and reducing sample volumes up to one-seventh during treatment, compared with the conventional method using bovine hemoglobin solution, resulting in extensive reduction of handling time. Phx-1 and Phx-3 thus obtained were identified as pure by the absorption spectra and NMR spectra. These phenoxazines exerted strong, dose-dependent anticancer activity against colon cancer cell lines COLO201, DLD1 and PMCO1 in vitro and induced apoptosis of these cells. The present results demonstrate that Phx-1 and Phx-3, which were prepared by extensively improved methods using bovine erythrocytes, may be useful as therapeutic drugs against colon cancer that is intractable to chemotherapy.