Forskolin-Induced Clearance of the Fluorescent Dye Sulforhodamine from Rat Parotid Intralobular Duct Lumen: Visualization of the Secretory Function under a Confocal Laser Scanning Microscope
Forskolin-Induced Clearance of the Fluorescent Dye Sulforhodamine from Rat Parotid Intralobular Duct Lumen: Visualization of the Secretory Function under a Confocal Laser Scanning Microscope
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毛喉素诱导大鼠腮腺小叶内导管腔中荧光染料磺胺罗丹明的清除：共焦激光扫描显微镜下分泌功能的可视化
DOI：
10.1007/s00232-002-1036-2
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Yoshiki Shiba
中科院分区：
文献类型：
--
作者：
T. Nakamoto;C. Hirono;M. Sugita;Kazuhisa Takemoto;Y. Iwasa;Yasumasa Akagawa;Yoshiki Shiba
环状AMP引起外分泌管中碳酸氢盐的液体分泌。在共聚焦激光扫描显微镜下进行光学切片，观察Forsklin对大鼠腮腺小叶内导管内荧光染料的清除作用，显示导管管腔内的荧光改变，以阐明导管的分泌功能。实验期间，用不透膜荧光染料灌流大鼠腮腺小叶内导管时，荧光染料被动进入导管间隙。福司可林和异丁基甲基黄嘌呤使阴离子染料磺胺若丹明B和中性染料右旋糖苷四甲基罗丹明在导管间隙的荧光减弱，提示福司可林诱导荧光染料的清除可能是导管内液体分泌的结果。甲唑胺可抑制Forsklin诱导的导管荧光持续下降和细胞内酸化。低浓度的外源性Cl2+、DIDS、布美他尼和阿米洛利不能明显抑制Forsklin诱导的导管荧光减弱。这些结果表明，Forskolin引起的导管荧光稳定下降的主要原因与细胞内HCO3？产生，而不是外源Cl2的吸收机制。格列本脲、NPPB、DPC和DMA均能抑制Forsklin诱导的降压作用。Forskolin引起荧光染料从导管空间的清除，可能是由于大鼠腮腺导管内的液体分泌，与通过与Na+/H+交换机制相连的碳酸氢酶依赖的碳酸氢盐的CFTR和DPC敏感的阴离子通道的分泌有关。
Cyclic AMP evokes fluid secretion with bicarbonate in exocrine ducts. Clearance of fluorescent dyes from rat parotid intralobular ducts by forskolin was visualized as a fluorescence change in the duct luminal space by optical sectioning under a confocal laser scanning microscope to clarify the secretory function in the ducts. When the isolated rat parotid intralobular duct segments were superfused with membrane-impermeable fluorescent dyes during the experimental period, fluorescent dyes were passively moved into the duct space. Forskolin and isobutylmethylxanthine decreased the fluorescence of anionic dye, sulforhodamine B, and neutral dye, dextran tetramethyl-rhodamine, in the duct space, suggesting that the forskolin-induced clearance of fluorescent dyes might be the result of fluid secretion in the ducts. Methazolamide inhibited a forskolin-induced sustained decrease in duct fluorescence and intracellular acidification. Low concentrations of external Cl?, DIDS, bumetanide and amiloride did not markedly inhibit a forskolin-induced decrease in duct fluorescence. These findings suggest that a major portion of the steady decrease in duct fluorescence by forskolin was related to intracellular HCO3? production, not the uptake mechanism of external Cl?. Glibenclamide, NPPB, DPC and DMA inhibited the forskolin-induced decrease. Forskolin evokes the clearance of fluorescent dyes from duct space possibly due to fluid secretion in rat parotid ducts, associated with secretion through CFTR and DPC-sensitive anion channels of carbonic anhydrase-dependent bicarbonate linked with the Na+/H+ exchange mechanism.
大鼠肾系膜细胞收缩诱导的 Na /H 交换激活。
DOI：
10.1152/ajpcell.1999.276.3.c674
发表时间：
1999
期刊：
The American journal of physiology
影响因子：
--
作者：
Bevensee,MO;Bashi,E;Schlue,WR;Boyarsky,G;Boron,WF
通讯作者：
Boron,WF
支气管粘膜下腺体的原位可视化及其对乙酰胆碱的分泌反应。
DOI：
10.1152/ajplung.1997.272.2.l203
发表时间：
1997
期刊：
The American journal of physiology
影响因子：
--
作者：
Inglis,SK;Corboz,MR;Taylor,AE;Ballard,ST
通讯作者：
Ballard,ST
DOI：
10.1073/pnas.94.7.3010
发表时间：
1997-04-01
影响因子：
11.1
作者：
Yun, CHC;Oh, S;Donowitz, M
通讯作者：
Donowitz, M