Synthesis, surface deposition, and secretion of immunoglobulins by abelson virus-transformed lymphosarcoma cell lines
Synthesis, surface deposition, and secretion of immunoglobulins by abelson virus-transformed lymphosarcoma cell lines
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阿贝尔森病毒转化淋巴肉瘤细胞系免疫球蛋白的合成、表面沉积和分泌
DOI：
10.1016/0092-8674(75)90005-7
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发表时间：
1975
期刊：
影响因子：
64.5
通讯作者：
M. Sklar
中科院分区：
文献类型：
--
作者：
E. Premkumar;M. Potter;P. Singer;M. Sklar
在组织培养中建立了三种阿贝尔森病毒转化的淋巴瘤细胞系，并研究了这些细胞系的免疫球蛋白生物合成。发现其中两种细胞系（ABLS-1 和 ABLS-5）能够合成沉积在细胞膜上的单体 IgM 分子，可能充当抗原受体。第三种细胞系 (ABLS-8) 被发现可以合成膜相关 IgM 以及细胞 IgG 分子。此外，这些细胞系被发现合成一种分子量为 35,000 的蛋白质，该蛋白质也是膜相关的，并且具有结合免疫球蛋白 (MAID) 的能力。据推测，该蛋白可能在使受体免疫球蛋白分子适应细胞膜的疏水环境中发挥作用。这些细胞系将氨基酸掺入免疫球蛋白的动力学表明，它们产生免疫球蛋白的速率比浆细胞瘤细胞（MOPC 104E）小两个数量级。这些结果表明，Abelson 病毒在不同的成熟阶段转化不依赖胸腺的淋巴细胞，并且这些淋巴细胞可能源自 B 细胞。用作对照细胞系的 T 淋巴瘤 (P1798) 偶尔会产生微量的免疫球蛋白。
Three Abelson virus-transformed lymphoma cell lines were established in tissue culture and the immunoglobulin biosynthesis by these cell lines was studied. Two of the cell lines (ABLS-1 and ABLS-5) were found to synthesize monomeric IgM molecules which were deposited in the cell membrane, probably to serve as an antigen receptor. The third cell line (ABLS-8) was found to synthesize membrane-associated IgM as well as cellular IgG molecules. In addition, these cell lines were found to synthesize a protein of 35,000 molecular weight which is also membrane-associated and which has the capability to bind the immunoglobulins(MAID). It is speculated that this protein might play a role in adapting the receptor lmmunoglobulin molecule to the hydrophobic environment of the cell membrane. The kinetics of amino acid incorporation into immunoglobulins by these cell lines show that they produce immunoglobulins at a rate which is two orders of magnitude smaller than plasmacytoma cells (MOPC 104E). These results suggest that Abelson virus transforms thymus-independent lymphocytes in vartous stages of maturation and these lymphocytes might be of B cell origin. The T lymphoma (P1798) used as a control cell line was found occasionally to produce minute amounts of immunoglobulin.