Sca-1pos cells in the mouse mammary gland represent an enriched progenitor cell population
Sca-1pos cells in the mouse mammary gland represent an enriched progenitor cell population
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DOI：
10.1006/dbio.2002.0625
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发表时间：
2002-05-01
影响因子：
2.7
通讯作者：
Goodell, MA
中科院分区：
文献类型：
--
作者：
Welm, BE;Tepera, SB;Goodell, MA
乳腺上皮在移植后可以功能性再生。这种更新能力通常归因于多能乳腺干细胞群的功能，该群被假设为乳腺癌病因学的主要目标。本研究采用了几种互补的方法来鉴定和富集保留干细胞特征的乳腺上皮细胞。使用长期 BrdU 标记，已鉴定出缺乏分化标记物表达的标记保留细胞 (LRC) 群体。从乳腺原代培养物中分离的 LRC 富含干细胞抗原 1 (Sca-1) 和 Hoechst 染料流出“侧群”特性。使用Sca-1(+/GFP)小鼠将乳腺中的Sca-1(pos)细胞定位于管腔上皮，其为孕酮受体阴性，并且不结合花生凝集素。最后，Sca-1(pox) 群体富含功能性干/祖细胞，移植到宿主小鼠清除的乳腺脂肪垫中时，与 Sca-1(neg) 细胞相比，其再生潜力增加就证明了这一点。 (C) 2002 年爱思唯尔科学（美国）。
Mammary epithelium can functionally regenerate upon transplantation. This renewal capacity has been classically ascribed to the function of a multipotent mammary gland stem cell population, which has been hypothesized to be a primary target in the etiology of breast cancer. Several complementary approaches were employed in this study to identify and enrich mammary epithelial cells that retain stem cell characteristics. Using long-term BrdU labeling, a population of label retaining cells (LRCs) that lack expression of differentiation markers has been identified. LRCs isolated from mammary primary cultures were enriched for stem cell antigen-1 (Sca-1) and Hoechst dye-effluxing "side population" properties. Sca-1(pos) cells in the mammary gland were localized to the luminal epithelia by using Sca-1(+/GFP) mice, were progesterone receptor-negative, and did not bind peanut lectin. Finally, the Sca-1(pox) population is enriched for functional stem/progenitor cells, as demonstrated by its increased regenerative potential compared with Sca-1(neg) cells when transplanted into the cleared mammary fat pads of host mice. (C) 2002 Elsevier Science (USA).