Influence of hydrogen-occluding-silica on migration and apoptosis in human esophageal cells in vitro.
Influence of hydrogen-occluding-silica on migration and apoptosis in human esophageal cells in vitro.
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DOI：
10.4103/2045-9912.208510
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发表时间：
2017-04
影响因子：
2.9
通讯作者：
Miwa N
中科院分区：
文献类型：
--
作者：
Li Q;Tanaka Y;Miwa N
在过去的十年中，许多研究表明，氢气或氢水可以降低活性氧的水平。分子氢具有抗氧化和抗凋亡作用，并对氧化应激诱导的细胞死亡具有预防作用。在本研究中，我们研究了固化的氢封闭二氧化硅（H2-二氧化硅），它可以释放分子氢到细胞培养基中，因为氢气的使用在医院和医疗设施和实验室中具有严格的处理限制，由于其物理化学特性。采用人食管鳞状细胞癌（KYSE-70）细胞和正常人食管上皮细胞（HEEPiCs）研究了H2-SiO2对细胞活力和增殖的影响。用伤口愈合和培养插入物迁移试验检查细胞迁移。细胞内活性氧的水平进行了评价与硝基四氮唑蓝测定。为了评估细胞的凋亡状态，通过蛋白质印迹分析Bax/Bcl-2比率和裂解的半胱天冬酶-3。结果显示，H2-SiO2给药对KYSE-70细胞和HEEPiCs普遍具有抑制作用，并且与对照组相比，在KYSE-70中存在显著的增殖抑制作用，并且具有H2-SiO2浓度依赖性（P < 0.05）。在10、300、600和1，200 ppm的H2-二氧化硅中观察到对KYSE-70细胞的凋亡诱导作用，并且仅1，200 ppm的H2-二氧化硅导致HEEpiC中的凋亡与对照组相比增加2.4倍，作为Bax/Bcl-2的指数。H2二氧化硅抑制KYSE-70细胞中的细胞迁移，并且高浓度对正常细胞具有细胞毒性作用。这些研究结果将提供深入了解H2-SiO2在体外抑制人类癌细胞的机制。
In the last decade, many studies have shown that hydrogen gas or hydrogen water can reduce the levels of reactive oxygen species in the living body. Molecular hydrogen has antioxidant and antiapoptotic effects and a preventive effect on oxidative stress-induced cell death. In the present study, we investigated solidified hydrogen-occluding-silica (H2-silica) that can release molecular hydrogen into cell culture medium because the use of hydrogen gas has strict handling limitations in hospital and medical facilities and laboratories, owing to its physicochemical characteristics. Human esophageal squamous cell carcinoma (KYSE-70) cells and normal human esophageal epithelial cells (HEEpiCs) were used to investigate the effects of H2-silica on cell viability and proliferation. Cell migration was examined with wound healing and culture-insert migration assays. The intracellular levels of reactive oxygen species were evaluated with a nitroblue tetrazolium assay. To assess the apoptotic status of the cells, the Bax/Bcl-2 ratio and cleaved caspase-3 were analyzed by western blot. The results showed that KYSE-70 cells and HEEpiCs were generally inhibited by H2-silica administration, and there was a significant proliferation-inhibitory effect in an H2-silica concentration-dependent manner compared with the control group (P < 0.05) in KYSE-70. Apoptosis-inducing effect on KYSE-70 cells was observed in 10, 300, 600, and 1,200 ppm H2-silica, and only 1,200 ppm H2-silica caused a 2.4-fold increase in apoptosis in HEEpiCs compared with the control group as the index of Bax/Bcl-2. H2 silica inhibited cell migration in KYSE-70 cells, and high concentrations had a cytotoxic effect on normal cells. These findings should provide insights into the mechanism of inhibition of H2-silica on human cancer cells in vitro.