Continuous electroencephalographic monitoring with radio-telemetry in a rat model of perinatal hypoxia-ischemia reveals progressive post-stroke epilepsy.
Continuous electroencephalographic monitoring with radio-telemetry in a rat model of perinatal hypoxia-ischemia reveals progressive post-stroke epilepsy.
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DOI：
10.1523/jneurosci.4093-09.2010
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发表时间：
2010-01-06
期刊：
影响因子：
--
通讯作者：
Dudek FE
中科院分区：
文献类型：
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作者：
Kadam SD;White AM;Staley KJ;Dudek FE
本实验研究了大鼠围产期缺氧缺血（HI）损伤后癫痫的发生。出生后第7天单侧颈动脉结扎缺氧后，连续无线电遥测和视频记录皮层脑电图和行为癫痫发作。慢性录音获得自由行为HI治疗和假手术对照大鼠2和12个月的年龄之间。假设癫痫与缺血性梗死直接相关（即，无损伤，无癫痫），并且所产生的癫痫是暂时进行性的。每只HI治疗的脑梗死大鼠均出现自发性癫痫样放电和复发性癫痫发作（100%）;相反，连续监测HI治疗的无梗死大鼠，未检测到自发性癫痫样放电或癫痫发作。2个月时的初始癫痫发作通常表现为局灶性发作，且无惊厥。随后的癫痫发作有局灶性发作，传播到同侧对侧皮质，并且是非惊厥性或部分性的;后来的癫痫发作通常表现为双侧发作，并且是惊厥性的。自发性癫痫样放电最初局限于同侧新皮层，但随着时间的推移变成双侧。自发性行为和电描记癫痫发作的严重程度和频率随时间逐渐增加。在每一只癫痫大鼠中，癫痫发作以不同的簇发生，无癫痫期长达几周。随着时间的推移，癫痫发作频率的逐渐增加与集群频率和每个集群内癫痫发作的增加有关。因此，长时间的连续癫痫监测直接表明，获得性癫痫后，围产期HI是渐进的癫痫发作集群，并始终与脑梗死。
The development of epilepsy after a perinatal hypoxic-ischemic (HI) insult was investigated in rats. After unilateral carotid ligation with hypoxia on postnatal day 7, cortical electroencephalographic and behavioral seizures were recorded with continuous radio-telemetry and video. Chronic recordings were obtained between 2 and 12 months of age in freely behaving HI-treated and sham-control rats. The hypotheses were that epilepsy is directly associated with an ischemic infarct (i.e., no lesion, no epilepsy), and the resultant epilepsy is temporally progressive. Every HI-treated rat with a cerebral infarct developed spontaneous epileptiform discharges and recurrent seizures (100%); in contrast, no spontaneous epileptiform discharges or seizures were detected with continuous monitoring in the HI-treated rats without infarcts. The initial seizures at 2 months generally showed focal onset and were non-convulsive. Subsequent seizures had focal onsets that propagated to the homotopic contralateral cortex and were non-convulsive or partial; later seizures often appeared to have bilateral onset and were convulsive. Spontaneous epileptiform discharges were initially lateralized to ipsilateral neocortex, but became bilateral over time. The severity and frequency of the spontaneous behavioral and electrographic seizures progressively increased over time. In every epileptic rat, seizures occurred in distinct clusters with seizure-free periods as long as a few weeks. The progressive increase in seizure frequency over time was associated with increases in cluster frequency and seizures within each cluster. Thus, prolonged, continuous seizure monitoring directly demonstrated that the acquired epilepsy after perinatal HI was progressive with seizure clusters and was consistently associated with a cerebral infarct.