Localization of actin and microfilament-associated proteins in the microvilli and terminal web of the intestinal brush border by immunofluorescence microscopy
Localization of actin and microfilament-associated proteins in the microvilli and terminal web of the intestinal brush border by immunofluorescence microscopy
复制标题
通过免疫荧光显微镜定位微绒毛和肠刷状缘末端网中的肌动蛋白和微丝相关蛋白
作者：
A. Bretscher;K. Weber
采用间接免疫荧光显微镜对小鼠肠上皮细胞刷状边缘的微丝相关蛋白进行定位。正如预期的那样，肌动蛋白抗体修饰了微绒毛的微丝，产生了非常强烈的荧光。相比之下，肌凝蛋白、原肌凝蛋白、丝蛋白和α -肌动蛋白的抗体没有修饰微绒毛。然而，所有这些抗体都修饰了刷状边缘的终网区域。肌凝蛋白，原肌凝蛋白和α -肌动蛋白，虽然存在于整个终网，但被发现优先位于细胞器周围。因此，两类微丝状结构可以记录在灌木边缘。首先，构成微绒毛核心的高度有序的微丝显然缺乏相关的蛋白质。其次，在肌凝蛋白、原肌凝蛋白、丝蛋白和α -肌动蛋白所在的末端网中发现了看似不那么有序的微丝。
Indirect immunofluorescence microscopy was used to localize microfilament-associated proteins in the brush border of mouse intestinal epithelial cells. As expected, antibodies to actin decorated the microfilaments of the microvilli, giving rise to a very intense fluorescence. By contrast, antibodies to myosin, tropomyosin, filamin, and alpha-actinin did not decorate the microvilli. All these antibodies, however, decorated the terminal web region of the brush border. Myosin, tropomyosin, and alpha-actinin, although present throughout the terminal web, were found to be preferentially located around the periphery of the organelle. Therefore, two classes of microfilamentous structures can be documented in the brush border. First, the highly ordered microfilaments which make up the cores of the microvilli apparently lack the associated proteins. Second, seemingly less-ordered microfilaments are found in the terminal web, in which region the myosin, tropomyosin, filamin and alpha-actinin are located.