The role of microstructured and interconnected pore channels in a collagen-based nerve guide on axonal regeneration in peripheral nerves
The role of microstructured and interconnected pore channels in a collagen-based nerve guide on axonal regeneration in peripheral nerves
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DOI：
10.1016/j.biomaterials.2011.10.069
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发表时间：
2012-02-01
期刊：
影响因子：
14
通讯作者：
Pallua, Norbert
中科院分区：
文献类型：
--
作者：
Bozkurt, Ahmet;Lassner, Franz;Pallua, Norbert
利用生物工程神经导片作为自体神经移植（ANT）的替代品是修复周围神经缺损的一种很有前途的策略。在本研究中，我们提出了一种基于胶原蛋白的微结构神经引导材料（periimaix）用于修复2cm大鼠坐骨神经缺损。Perimaix是一种开放多孔的可生物降解的神经导管，含有连续的纵向定向通道，用于定向神经生长。在植入后6周，我们比较了这些神经引导剂对轴突再生的影响。对再生坐骨神经的研究表明，Perimaix强烈支持定向轴突再生。当用培养的大鼠雪旺细胞（SC）播种时，发现周围神经引导物几乎与ANT一样支持轴突再生。使用转基因绿色荧光蛋白（GFP）大鼠的SC使我们能够在移植后1周和6周检测供体SC的活力。gfp阳性SC在纵向定向的微通道内呈柱状排列。这种细胞排列不仅在植入前观察到，而且在植入后1周和6周也观察到。结论：周围神经导片在周围神经缺损的修复中具有广阔的应用前景。(C) 2011 Elsevier Ltd.版权所有。
The use of bioengineered nerve guides as alternatives for autologous nerve transplantation (ANT) is a promising strategy for the repair of peripheral nerve defects. In the present investigation, we present a collagen-based micro-structured nerve guide (Perimaix) for the repair of 2 cm rat sciatic nerve defects. Perimaix is an open-porous biodegradable nerve guide containing continuous, longitudinally orientated channels for orientated nerve growth. The effects of these nerve guides on axon regeneration by six weeks after implantation have been compared with those of ANT. Investigation of the regenerated sciatic nerve indicated that Perimaix strongly supported directed axon regeneration. When seeded with cultivated rat Schwann cells (SC), the Perimaix nerve guide was found to be almost as supportive of axon regeneration as ANT. The use of SC from transgenic green-fluorescent-protein (GFP) rats allowed us to detect the viability of donor SC at 1 week and 6 weeks after transplantation. The GFP-positive SC were aligned in a columnar fashion within the longitudinally orientated micro-channels. This cellular arrangement was not only observed prior to implantation, but also at one week and 6 weeks after implantation. It may be concluded that Perimaix nerve guides hold great promise for the repair of peripheral nerve defects. (C) 2011 Elsevier Ltd. All rights reserved.