PC12 cells stimulate vascular smooth muscle growth.
PC12 cells stimulate vascular smooth muscle growth.
复制标题
PC12 细胞刺激血管平滑肌生长。
DOI：
10.1097/00005344-200110000-00015
复制
发表时间：
2001
影响因子：
3
通讯作者：
Damon,DH
中科院分区：
文献类型：
--
作者：
Damon,DH
交感神经刺激血管生长。这种刺激的机制尚未完全阐明。用PC 12细胞和血管平滑肌培养物研究交感神经刺激对血管平滑肌生长的影响。未分化和分化的PC 12细胞条件培养液刺激血管平滑肌生长（446±47%）。分化的PC 12细胞比未分化的PC 12细胞产生更多的生长刺激活性（61.5±9.6/10 6细胞）（28.5±8.8/10 6细胞）。肾上腺素能受体拮抗剂不能抑制PC 12对血管平滑肌生长的刺激，但内皮素拮抗剂苏拉明和中和血小板衍生生长因子活性的抗体可降低PC 12对血管平滑肌生长的刺激。这些数据表明，内皮素和血小板源性生长因子，但不是儿茶酚胺，在血管平滑肌生长的交感神经刺激中发挥作用。
Sympathetic nerves stimulate vascular growth. The mechanisms underlying this stimulation have not been fully elucidated. PC12 cells and cultures of vascular smooth muscle were used to study sympathetic stimulation of vascular smooth muscle growth. Media conditioned by undifferentiated and differentiated PC12 cells stimulated the growth of vascular smooth muscle (446±47%). Differentiated PC12 cells produced more growth-stimulatory activity (61.5±9.6 per 10 6 cells) than undifferentiated PC12 cells (28.5±8.8 per 10 6 cells). PC12 stimulation of vascular smooth muscle growth was not inhibited by adrenergic receptor antagonists but was reduced by an endothelin antagonist, suramin, and an antibody that neutralized the activity of platelet-derived growth factor. These data suggest that endothelin and platelet-derived growth factor, but not catecholamines, play a role in sympathetic stimulation of vascular smooth muscle growth.