env Sequences of simian immunodeficiency viruses from chimpanzees in Cameroon are strongly related to those of human immunodeficiency virus group N from the same geographic area
env Sequences of simian immunodeficiency viruses from chimpanzees in Cameroon are strongly related to those of human immunodeficiency virus group N from the same geographic area
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DOI：
10.1128/jvi.74.1.529-534.2000
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发表时间：
2000-01-01
影响因子：
5.4
通讯作者：
Mauclere, P
中科院分区：
文献类型：
--
作者：
Corbet, S;Müller-Trutwin, MC;Mauclere, P
来自喀麦隆的人类免疫缺陷病毒 1 型 (HIV-1) N 组在环境发育上与来自加蓬的猿猴免疫缺陷病毒 (SIV) cpz-gab 和地理起源未知的 SIVcpz-US 相近。我们筛查了 29 只野生喀麦隆黑猩猩，通过蛋白质印迹发现其中 3 只（Cam3、Cam4 和 Cam5）HIV-1 呈阳性。线粒体DNA序列分析表明Cam3和Cam5属于Pan troglodytes troglodytes，Cam4属于P. t. troglodytes。毳骨。对病毒的遗传分析以及血清学数据表明，两种 P. t. 中至少有一种。穴居黑猩猩 (Cam5) 在野外被感染，并揭示了 Cam3 和 Cam4 之间的水平传播。这些数据证实了 P. t.穴居人是 HIV-1 相关病毒的天然宿主。此外，他们还表明，SIVcpz 可以在圈养条件下从一个黑猩猩亚种传播到另一个亚种。所有三种 SIVcpz-cam 病毒在环境中都与 HIV-1 N 聚集。完整的 Cam3 SIVcpz 基因组序列显示与 SIVcpz-US（一种在 P t 中鉴定的病毒）非常密切的系统发育关系。近 40 年前捕获的穴居人黑猩猩。与 SIVcpz-US 一样，SIVcpz-cam3 在 env 中与 HIV-1 N 密切相关，但在 pol 中则不然，这支持了 HIV-1 N 由重组事件引起的假设。因此，来自喀麦隆野外出生的黑猩猩的 SIVcpz 在环境方面与来自喀麦隆的 HIV-1 N 密切相关，这证明了这些人类和猿猴病毒在地理上的巧合，并为支持 HIV-1 起源于黑猩猩提供了进一步有力的论据。
Human immunodeficiency virus type 1 (HIV-1) group N from Cameroon is phylogenetically close, in env, to the simian immunodeficiency virus (SIV) cpz-gab from Gabon and SIVcpz-US of unknown geographic origin. We screened 29 wild-born Cameroonian chimpanzees and found that three (Cam3, Cam4, and Cam5) were positive for HIV-1 by Western blotting. Mitochondrial DNA sequence analysis demonstrated that Cam3 and Cam5 belonged to Pan troglodytes troglodytes and that Cam4 belonged to P. t. vellerosus. Genetic analyses of the viruses together with serological data demonstrated that at least one of the two P. t. troglodytes chimpanzees (Cam5) was infected in the wild, and revealed a horizontal transmission between Cam3 and Cam4. These data confirm that P. t. troglodytes is a natural host for HIV-1-related viruses. Furthermore, they show that SIVcpz can be transmitted in captivity, from one chimpanzee subspecies to another. All three SIVcpz-cam viruses clustered with HIV-1 N in env. The full Cam3 SIVcpz genome sequence showed a very close phylogenetic relationship with SIVcpz-US, a virus identified in a P t. troglodytes chimpanzee captured nearly 40 years earlier. Like SIVcpz-US, SIVcpz-cam3 was closely related to HIV-1 N in env, but not in pol, supporting the hypothesis that HIV-1 N results from a recombination event. SIVcpz from chimpanzees born in the wild in Cameroon are thus strongly related in env to HIV-1 N from Cameroon, demonstrating the geographic coincidence of these human and simian viruses and providing a further strong argument in favor of the origin of HIV-1 being in chimpanzees.