In vitro suppression of fungi caused by combinations of apparently non-antagonistic soil bacteria
In vitro suppression of fungi caused by combinations of apparently non-antagonistic soil bacteria
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DOI：
10.1111/j.1574-6941.2006.00197.x
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发表时间：
2007-01-01
影响因子：
4.2
通讯作者：
van Veen, Johannes A.
中科院分区：
文献类型：
--
作者：
de Boer, Wietse;Wagenaar, Anne-Marieke;van Veen, Johannes A.
我们假设，显然非拮抗性土壤细菌可能有助于抑制真菌与其他细菌的竞争性相互作用。4种土壤细菌（Brevundimonas sp.，黄杆菌属，和假单胞菌属）在不同的琼脂培养基上表现出很少或没有可见的抗真菌活性。这些物种的单一和混合菌株进行了测试，对植物病原真菌镰刀菌culmorum和立枯丝核菌和腐食性真菌哈茨木霉的营养不良的琼脂培养基上的拮抗作用。单一细菌菌株导致真菌的生长减少（定量为麦角固醇）很少到中度，最可能是由于从培养基中提取营养。混合菌群对真菌的抑制作用比单一菌株强得多。这似乎主要是由于假单胞菌和土壤杆菌菌株之间的竞争性相互作用。我们认为，这些菌株的同居通过种间相互作用引发抗生素的产生，真菌的生长减少是由真菌对细菌次级代谢产物的敏感性引起的副作用。还观察到其他菌株对土壤杆菌属菌株中的滑动行为的诱导。我们的研究结果表明，显然非拮抗性土壤细菌可能是重要的贡献者，土壤抑制和抑菌作用时，在一个社区的背景下。
We hypothesized that apparently non-antagonistic soil bacteria may contribute to suppression of fungi during competitive interactions with other bacteria. Four soil bacteria (Brevundimonas sp., Luteibacter sp., Pedobacter sp. and Pseudomonas sp.) that exhibited little or no visible antifungal activity on different agar media were prescribed. Single and mixed strains of these species were tested for antagonism on a nutrient-poor agar medium against the plant pathogenic fungi Fusarium culmorum and Rhizoctonia solani and the saprotrophic fungus Trichoderma harzianum. Single bacterial strains caused little to moderate growth reduction of fungi (quantified as ergosterol), most probably due to nutrient withdrawal from the media. Growth reduction of fungi by the bacterial mixture was much stronger than that by the single strains. This appeared to be mostly due to competitive interactions between the Pseudomonas and Pedobacter strains. We argue that cohabitation of these strains triggered antibiotic production via interspecific interactions and that the growth reduction of fungi was a side-effect caused by the sensitivity of the fungi to bacterial secondary metabolites. Induction of gliding behavior in the Pedobacter strain by other strains was also observed. Our results indicate that apparently non-antagonistic soil bacteria may be important contributors to soil suppressiveness and fungistasis when in a community context.