INTEGRATED OPTICAL IMMUNOSENSOR FOR S-TRIAZINE DETERMINATION - REGENERATION, CALIBRATION AND LIMITATIONS
INTEGRATED OPTICAL IMMUNOSENSOR FOR S-TRIAZINE DETERMINATION - REGENERATION, CALIBRATION AND LIMITATIONS
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DOI：
10.1039/an9941900437
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发表时间：
1994-03-01
期刊：
影响因子：
4.2
通讯作者：
SCHMID, RD
中科院分区：
文献类型：
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作者：
BIER, FF;JOCKERS, R;SCHMID, RD
光栅耦合器，作为一个集成的光学传感器的一个例子，进行了研究，用于无标记免疫传感的流通系统。使用三嗪（特丁净）作为一个例子，证明了通过竞争性测定格式的农药检测。通过固定半抗原实现了长期稳定性和再现性，在这种情况下，半抗原是待测农药的衍生物。因此，传感器的寿命不取决于抗体的稳定性，而仅受固定质量的限制。再生的传感器表面实现使用蛋白酶支持的酸性洗涤，它是直接观察，因此可以优化。因此，在免疫传感中通常至关重要的校准是可能的。非特异性结合测定为10%，可通过加入牛血清白蛋白降低。发现的检测限为特丁净是15杯L-1的安排中使用的，提高灵敏度的策略进行了讨论。
Grating couplers, as an example of an integrated optical transducer, were investigated for label-free immunosensing in a flow-through system. The detection of pesticides by a competitive assay format was demonstrated using a triazine (terbutryn) as an example. Long-term stability and reproducibility were achieved by immobilizing the hapten, which in this instance was a derivative of the pesticide to be determined. Hence, the lifetime of the sensor does not depend on the stability of the antibody but is limited only by the quality of the immobilization. Regeneration of the sensor surface was achieved using proteinase supported by acidic washing; it was observed directly and could therefore be optimized. In consequence, calibration, usually critical in immunosensing, was possible. Non-specific binding was determined to be 10% and could be decreased by addition of bovine serum albumin. The detection limit found for terbutryn was 15 mug l-1 in the arrangement used; strategies for enhancement of sensitivity are discussed.