Messenger RNA expression of periostin and Twist transiently decrease by occlusal hypofunction in mouse periodontal ligament
Messenger RNA expression of periostin and Twist transiently decrease by occlusal hypofunction in mouse periodontal ligament
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DOI：
10.1016/j.archoralbio.2005.04.002
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发表时间：
2005-12-01
影响因子：
3
通讯作者：
Moriyama, K
中科院分区：
文献类型：
--
作者：
Afanador, E;Yokozeki, M;Moriyama, K
骨膜蛋白是一种支持细胞粘附的分泌性蛋白，在牙周组织中高度表达。韧带（PDL）。Twist是一种碱性螺旋-环-螺旋（bHLH）转录因子，是成骨细胞分化的负调控因子，可调控骨膜蛋白基因的转录。由于咬合力被认为是重要的牙周膜的动态平衡，在这项研究中，我们研究了骨膜蛋白和Twist mRNA在小鼠牙周组织中的表达去除后。对抗性的牙齿单方面的。在3周龄的交配小鼠中进行上颌牙齿拔除以产生右下颌磨牙的咬合功能减退。采用真实的免疫组化法检测骨膜蛋白和Twist mRNA的表达。分别于拔牙后12、24、72、168 h进行time-PCR和原位杂交。实时荧光定量PCR分析显示，骨膜蛋白和Twist mRNA在24 h时分别为对照组的14.5%和49.9%。但在72和168 h开始恢复，没有观察到显著差异。如通过原位杂交分析所确定的，骨膜蛋白和Twist mRNA表达PDL细胞的数量在24 h显示出显著减少，尽管从72 h开始观察到增加，直到168 h时的分布几乎与对照组相似。这些结果表明，咬合力可能与牙周膜中periostin和Twist基因的表达有关，其表达水平的变化可能被认为是对环境变化的一种适应。(c)2005 Elsevier Ltd. All. rights reserved.
Periostin, which is a secreted protein that supports cell adhesion, is highly expressed in the periodontal. ligament (PDL). Twist, a basic helix-loop-helix (bHLH) transcription factor and a negative regulator of osteobtast differentiation, has been found to regulate the periostin gene transcription. Since occlusal force is thought to be important in the homeostasis of the PDL, in this study we investigated the expression of periostin and Twist mRNA in the mouse periodontal tissue following removal. of antagonizing teeth. Unilateral. maxillary tooth extraction was performed in 3-week-old mate mice to produce occlusal hypofunction of the right mandibular molars. The expressions of periostin and Twist mRNA were examined by real. time-PCR and in situ hybridization at 12, 24, 72 and 168 h after the tooth extraction. The real-time PCR analysis showed that periostin and Twist mRNA significantly decreased at 24 h to 14.5 and 49.9% of those in control group, respectively. But the recovery began at 72 and 168 h, no significant difference was observed. As determined by in situ hybridization analysis, the number of periostin and Twist mRNA-expressing PDL cells showed a marked decrease at 24 h, although an increase was observed from 72 h until the distribution was almost similar to that of the control group at 168 h. These results suggested that occlusal force might have putative rotes in periostin and Twist gene expression in the PDL and the changes in their expression level during hypofunction may be considered a form of adaptation to environmental changes. (c) 2005 Elsevier Ltd. All. rights reserved.