Electrokinetic focusing in microfabricated channel structures
Electrokinetic focusing in microfabricated channel structures
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DOI：
10.1021/ac961093z
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发表时间：
1997-08-15
影响因子：
7.4
通讯作者：
Ramsey, JM
中科院分区：
文献类型：
--
作者：
Jacobson, SC;Ramsey, JM
制造玻璃微芯片以展示使用动电力的离子和流体的二维空间限制。中性和带电的分子样品可以在18 μ m长× 18 μ m宽的室中电动聚焦到3.3 μ m的宽度。使用阳离子、中性和阴离子样品测试电动聚焦特性，以评估电场对样品流的影响。使用电泳、电渗或两者的组合来实现聚焦。此外，通过采用样品堆叠，在聚焦室中观察到浓度增强，并且聚焦室被复用以在空间上同时限制多于一个的样品流。
Glass microchips are fabricated to demonstrate two-dimensional spatial confinement of ions and fluids using electrokinetic forces. Neutral and charged molecular samples can be electrokinetically focused to widths as narrow as 3.3 mu m in a 18 mu m long by 18 mu m wide chamber. The electrokinetic focusing characteristics are tested using cationic, neutral, and anionic samples to evaluate the influence of electric fields on the sample stream. Focusing is achieved using electrophoresis, electroosmosis, or a combination of the two. In addition, concentration enhancement is observed in the focusing chamber by employing sample stacking, and the focusing chamber is multiplexed to confine spatially more than one sample stream simultaneously.