Bee-Vectored Aureobasidium pullulans for Biological Control of Gray Mold in Strawberry
Bee-Vectored Aureobasidium pullulans for Biological Control of Gray Mold in Strawberry
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DOI：
10.1094/phyto-05-21-0205-r
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发表时间：
2022-02-01
期刊：
影响因子：
3.2
通讯作者：
Stenberg, Johan A.
中科院分区：
文献类型：
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作者：
Iqbal, Mudassir;Jutzeler, Matilda;Stenberg, Johan A.
灰霉病是草莓采后常见的一种病害，它会大大降低草莓的保质期。以大黄蜂（Bombus terrestris）为载体，在Flying DoctorsVR系统中研究了酵母样生物防治真菌普鲁兰金黄色葡萄球菌（AP-SLU6）抑制病原菌和延长收获草莓（Sonata品种）保质期的潜力。使用大黄蜂作为各种生物防治剂的载体正变得越来越流行，但对蜜蜂性能的潜在负面影响尚未得到充分研究。我们的研究结果表明，在4周的试验期间，蜜蜂的性能和活动没有受到普鲁兰的负面影响。蜜蜂成功地捡起了粉末配方；然后，他们把它搬到花上。与对照处理相比，生物防治剂的使用显著减少了果实灰霉病的发生45%，使贮藏期延长100%。说明开花期间施用的生防菌成功地减少了葡萄孢菌的侵染，从而有效地保护了果实免受灰霉病的侵害。此外，研究发现，以蜜蜂为媒介施用生物防治剂明显比喷雾施用更有效，因为后者可能暂时增加花周围的湿度，从而为病原体的繁殖创造适宜的环境。综上所述，本研究表明，在不影响蜜蜂的情况下，大黄蜂载体普鲁兰可减少草莓灰霉病的感染，提高草莓的保质期，从而为草莓灰霉病的可持续有效防治提供依据。
Gray mold caused by Botrytis cinerea is a common postharvest disease in strawberries, reducing shelf life considerably. We investigated the potential of the yeast-like biocontrol fungus Aureobasidium pullulans (AP-SLU6) vectored by bumblebees (Bombus terrestris) in the Flying DoctorsVR system to inhibit the pathogen and increase the shelf life of harvested strawberries (cultivar Sonata). Using bumblebees as vectors of various biocontrol agents is becoming increasingly popular, but any potentially negative effects on bee performance have been understudied. Our results show that, over the 4-week period of the trial, the performance and activity of the bees were not negatively affected by A. pullulans. The bees successfully picked up the powder formulation; then, they carried and deposited it on the flowers. The vectoring of the biocontrol agent significantly reduced gray mold development on the harvested fruits by 45% and increased shelf life by 100% in comparison with control treatments. This suggests that the biocontrol fungus applied during flowering successfully reduced Botrytis infection and thus, effectively protected the fruits from gray mold. In addition, the bee-vectored application of the biocontrol agent was found to be significantly more effective than spray application because the latter may temporarily increase humidity around the flower, thereby creating a suitable environment for the pathogen to thrive. In summary, our study demonstrates that A. pullulans vectored by bumblebees can decrease gray mold infection and improve the shelf life of strawberries without adversely affecting the bees, thus providing a basis for the sustainable and efficient control of gray mold on strawberry.