Human colostrum: Identification of minor proteins in the aqueous phase by proteomics
Human colostrum: Identification of minor proteins in the aqueous phase by proteomics
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DOI：
10.1002/pmic.200500558
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发表时间：
2006-04-01
期刊：
影响因子：
3.4
通讯作者：
Cooper, GJ
中科院分区：
文献类型：
--
作者：
Palmer, DJ;Kelly, VC;Cooper, GJ
人初乳是新生儿保护、营养和发育因子的重要来源。我们已经调查了低丰度蛋白质在水相的人初乳，消耗后的主要蛋白质分泌伊加，乳铁蛋白，α-乳白蛋白和HSA的免疫吸附，使用2-D LC和凝胶为基础的蛋白质组学方法。鉴定了151种蛋白质，其中83种以前在人类初乳或牛奶中没有报道过。这是第一个全面的蛋白质组学分析的人初乳中产生的第一个48小时的哺乳期。
Human colostrum is an important source of protective, nutritional and developmental factors for the newborn. We have investigated the low abundance proteins in the aqueous phase of human colostrum, after depletion of the major proteins secretory IgA, lactoferrin, alpha-lactalbumin and HSA by immunoabsorption, using 2-D LC and gel-based proteomic methods. One hundred and fifty-one proteins were identified, 83 of which have not been previously reported in human colostrum, or milk. This is the first comprehensive proteomic analysis of human colostrum produced during the first 48 h of lactation.