ALTERING THE INSERTIONAL SPECIFICITY OF A DROSOPHILA TRANSPOSABLE ELEMENT
ALTERING THE INSERTIONAL SPECIFICITY OF A DROSOPHILA TRANSPOSABLE ELEMENT
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DOI：
10.1073/pnas.89.5.1919
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发表时间：
1992-03-01
影响因子：
11.1
通讯作者：
PERRIMON, N
中科院分区：
文献类型：
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作者：
KASSIS, JA;NOLL, E;PERRIMON, N
由果蝇P元件转座子衍生的载体被广泛用于转基因果蝇。大多数p元素衍生向量的插入是非随机的，但它们对目标位点具有广泛的特异性。在鉴定果蝇片段基因的顺式调控元件的实验中，我们鉴定了一段嵌入的DNA片段，当它被包含在p元素载体中时，会显著改变目标位点的特异性。p -元件载体包含这段编码的调控DNA片段，以高频率插入条带表达的基因附近。
Vectors derived from the Drosophila P element transposon are widely used to make transgenic Drosophila. Insertion of most P-element-derived vectors is nonrandom, but they exhibit a broad specificity of target sites. During experiments to identify cis-acting regulatory elements of the Drosophila segmentation gene engrailed, we identified a fragment of engrailed DNA that, when included within a P-element vector, strikingly alters the specificity of target sites. P-element vectors that contain this fragment of engrailed regulatory DNA insert at a high frequency near genes expressed in stripes.