Islet regeneration during the reversal of autoimmune diabetes in NOD mice
Islet regeneration during the reversal of autoimmune diabetes in NOD mice
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DOI：
10.1126/science.1088949
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发表时间：
2003-11-14
期刊：
影响因子：
56.9
通讯作者：
Faustman, DL
中科院分区：
文献类型：
--
作者：
Kodama, S;Kühtreiber, W;Faustman, DL
非肥胖糖尿病（NOD）小鼠是人类1型糖尿病的模型。通过注射供体脾细胞和完全弗氏佐剂治疗患有终末期疾病的NOD小鼠，消除了自身免疫并永久恢复正常免疫力。内源性胰岛素分泌的恢复伴随着胰腺β细胞的重新出现。我们现在表明，给予糖尿病NOD女性的活供体男性或标记的脾细胞含有快速分化为胰腺内胰岛和导管上皮细胞的细胞。用辐照的脾细胞处理后也可以再生胰岛，但速度较慢。在这两种情况下产生的胰岛是持久的，功能性的，在所有NOD宿主中是明显的，具有永久性疾病逆转。
Nonobese diabetic (NOD) mice are a model for type 1 diabetes in humans. Treatment of NOD mice with end-stage disease by injection of donor splenocytes and complete Freund's adjuvant eliminates autoimmunity and permanently restores normoglycemia. The return of endogenous insulin secretion is accompanied by the reappearance of pancreatic beta cells. We now show that live donor male or labeled splenocytes administered to diabetic NOD females contain cells that rapidly differentiate into islet and ductal epithelial cells within the pancreas. Treatment with irradiated splenocytes is also followed by islet regeneration, but at a slower rate. The islets generated in both instances are persistent, functional, and apparent in all NOD hosts with permanent disease reversal.