Berberine inhibits LPS-induced TF procoagulant activity and expression through NF-κB/p65, Akt and MAPK pathway in THP-1 cells
Berberine inhibits LPS-induced TF procoagulant activity and expression through NF-κB/p65, Akt and MAPK pathway in THP-1 cells
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DOI：
10.1016/j.pharep.2013.12.004
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发表时间：
2014-06-01
影响因子：
4.4
通讯作者：
Yu, Bo-yang
中科院分区：
文献类型：
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作者：
Gao, Meng-yu;Chen, Ling;Yu, Bo-yang
背景：考虑到TF在脓毒症或急性肺损伤（acute lung injury， ALL）凝血中的关键作用，我们研究了小檗碱（berberine， BBR）是否能抑制TF的表达和促凝活性，并探讨其可能的机制。方法：观察小檗碱对THP-1细胞中脂多糖（LPS）诱导的TF表达、促凝活性及相关信号通路的影响。结果：我们的研究结果显示，小檗碱可以抑制lps诱导的TF活性和表达，下调NF-kappa B、Akt和MAPK/JNK/p38/ERK通路。结论：小檗碱抑制TF表达及相关通路，为其治疗败血症的机制提供了一些新的认识。(C) 2014年波兰科学院药理学研究所。Elsevier Urban & Partner Sp. z . oo出版，版权所有。
Background: Considering the key role of TF in coagulation of sepsis or acute lung injury (ALL), we investigated whether berberine (BBR) could inhibit TF expression and procoagulant activity and explored its possible mechanism.Methods: The effects of berberine on the expression, procoagulant activity of TF and related signal pathways induced by lipopolysaccharide (LPS) were observed in THP-1 cells.Results: Our results showed that berberine could inhibit LPS-induced TF activity and expression, and down-regulate NF-kappa B, Akt and MAPK/JNK/p38/ERK pathways.Conclusion: Berberine inhibits TF expression and related pathway, which provides some new insights on its mechanism for sepsis treatment. (C) 2014 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.