Application of a Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for the Determination of Ractopamine in Incurred Samples from Food Animals
Application of a Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for the Determination of Ractopamine in Incurred Samples from Food Animals
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DOI：
10.1021/jf011372
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发表时间：
2002-02
影响因子：
6.1
通讯作者：
W. Shelver;David J. Smith
中科院分区：
文献类型：
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作者：
W. Shelver;David J. Smith
基于单克隆抗体的莱克多巴胺免疫测定法已应用于绵羊和牛的真实样品。将免疫测定法获得的结果与高效液相色谱法（HPLC）获得的结果进行比较。分析了三组主要含有未代谢莱克多巴胺的样品提取物。尽管莱克多巴胺浓度相当低（范围1.1 - 13.4 ng/mL，n = 6），但牛肝样品的HPLC与酶联免疫吸附测定（ELISA）的相关性得出r2 = 0.98。通过固相萃取去除莱克多巴胺代谢物处理的牛尿液样品中的莱克多巴胺浓度也显示出HPLC和ELISA结果之间的高度相关性（r2 = 0.95，范围1.0 - 275 ng/mL，n = 61）。相比之下，羊尿中莱克多巴胺的HPLC和ELISA分析相关性不好（r2 = 0.58，范围0.85 - 51 ng/mL，n = 34）。用水解酶水解尿样中的莱克多巴胺结合物，ELISA法与HPLC法的相关性很高[羊（范围：0.94），
A monoclonal antibody-based ractopamine immunoassay has been applied to incurred samples from sheep and cattle. Results obtained by immunoassay were compared with those from high-performance liquid chromatography (HPLC). Three sets of sample extracts containing primarily unmetabolized ractopamine were analyzed. Correlation of HPLC with enzyme-linked immunosorbent assay (ELISA) for beef liver samples gave an r2 = 0.98 despite rather low ractopamine concentrations (range 1.1−13.4 ng/mL, n = 6). Ractopamine concentrations in cow urine samples treated by solid phase extraction, to remove ractopamine metabolites, also showed a high correlation between the HPLC and the ELISA results (r2 = 0.95, range 1.0−275 ng/mL, n = 61). In contrast, HPLC and ELISA analyses of ractopamine in sheep urine were not well-correlated (r2 = 0.58, range 0.85−51 ng/mL, n = 34). When ractopamine conjugates in urine samples were hydrolyzed with hydrolytic enzymes, ELISA and HPLC methods were highly correlated [r2 = 0.94 for sheep (rang...