CONSEQUENCES OF RESTRAINT STRESS ON NATURAL-KILLER-CELL ACTIVITY, BEHAVIOR, AND HORMONE LEVELS IN RHESUS MACAQUES (MACACA-MULATTA)
CONSEQUENCES OF RESTRAINT STRESS ON NATURAL-KILLER-CELL ACTIVITY, BEHAVIOR, AND HORMONE LEVELS IN RHESUS MACAQUES (MACACA-MULATTA)
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DOI：
10.1016/0306-4530(93)90013-b
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发表时间：
1993-01-01
影响因子：
3.7
通讯作者：
NORMAN, RL
中科院分区：
文献类型：
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作者：
MORROWTESCH, JL;MCGLONE, JJ;NORMAN, RL
为了确定压力对非人类灵长类动物神经内分泌免疫系统的影响，进行了三个实验。实验1测定细胞和激素水平的日变化。中性粒细胞、单核细胞和嗜酸性粒细胞的百分比在24小时内波动，而白细胞（WBC）、中性粒细胞与淋巴细胞比率（N:L）、血红蛋白（Hgb）、自然杀伤细胞毒性（NK活性）和β -内啡肽水平则无波动。实验2研究氯胺酮和约束对行为的影响。对照组的猴子和接受氯胺酮治疗的动物抓挠的次数增加，而坐在椅子上的动物的被动反应增加。在实验3中，8只成年雄性恒河猴于0600h被限制在灵长类动物椅子上。记录行为3小时，并于0700、0800和0900采集血样。分析全血白细胞总数和各白细胞类型的百分比。同时测量NK活性。血浆皮质醇和-内啡肽水平被测定，行为被定量地记录下来。抑制期白细胞和中性粒细胞百分比升高，淋巴细胞和单核细胞百分比下降。NK活性也随着时间的推移而降低，而血浆皮质醇和β -内啡肽水平显著升高。虽然在3小时的约束应激后，发现激素水平、行为和NK活性发生了变化，但所测参数之间没有显著相关性。因此，我们的研究结果表明，没有一个共同的神经内分泌反应或单一的神经内分泌介质导致可预测的行为改变和应激后的免疫抑制。
Three experiments were performed to determine the effect of stress on the neuroendocrine-immune system in nonhuman primates. In Experiment 1 the diurnal variation in cell and hormone levels was determined. The percentages of neutrophils, monocytes, and eosinophils fluctuated throughout the 24-hr period, while white blood cell (WBC), neutrophil-to-lymphocyte ratio (N:L), hemoglobin (Hgb), natural killer cell cytotoxicity (NK activity) an beta-endorphin levels did not. Experiment 2 investigated the effects of ketamine and restraint on behavior. Scratching was increased in control monkeys and animals receiving ketamine, whereas passivity was increased in chair-restrained animals. In Experiment 3, eight adult male rhesus monkeys were restrained in primate chairs at 0600h. Behavior was filmed for 3 hr and blood samples were collected at 0700, 0800, and 0900. Whole blood was analyzed for total WBC and percentage of each leukocyte type. NK activity was also measured. Plasma levels of cortisol and beta-endorphin were determined and behavior was quantitated from video-records. WBC and the percentage of neutrophils increased during the restraint period, while the percent lymphocytes and monocytes decreased. NK activity also decreased over time after restraint whereas plasma cortisol and beta-endorphin levels increased significantly. Although after the 3 hr of restraint stress, changes were found in hormone levels, behavior, and NK activity, there were no significant correlations between the parameters measured. Thus, our results indicate that there is not a common neuroendocrine response or single neuroendocrine mediator that results in predictable behavioral changes and immune suppression following stress.