Multi-layered inhibition of Streptomyces development: BldO is a dedicated repressor of whiB.
Multi-layered inhibition of Streptomyces development: BldO is a dedicated repressor of whiB.
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DOI：
10.1111/mmi.13663
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发表时间：
2017-06
影响因子：
3.6
通讯作者：
Buttner MJ
中科院分区：
文献类型：
--
作者：
Bush MJ;Chandra G;Findlay KC;Buttner MJ
BldD-(c-di-GMP) 位于控制链霉菌分化的调节网络之上，当细菌营养生长时，抑制发育基因的大量调节子。通过这种方式，BldD 起到抑制剂的作用，阻止孢子形成的启动。在此，我们报告了 BldO 的鉴定和表征，BldO 是一种额外的发育抑制因子，可维持营养生长并防止进入孢子形成。然而，与多效性调节因子 BldD 不同，我们发现 BldO 作为单个关键靶基因 whiB 的专用阻遏蛋白发挥作用，并且 bldO 的缺失或 whiB 的组成型表达足以诱导早熟超孢子形成。
BldD‐(c‐di‐GMP) sits on top of the regulatory network that controls differentiation in Streptomyces, repressing a large regulon of developmental genes when the bacteria are growing vegetatively. In this way, BldD functions as an inhibitor that blocks the initiation of sporulation. Here, we report the identification and characterisation of BldO, an additional developmental repressor that acts to sustain vegetative growth and prevent entry into sporulation. However, unlike the pleiotropic regulator BldD, we show that BldO functions as the dedicated repressor of a single key target gene, whiB, and that deletion of bldO or constitutive expression of whiB is sufficient to induce precocious hypersporulation.