Enteral Tube Feeding Alters the Oral Indigenous Microbiota in Elderly Adults
Enteral Tube Feeding Alters the Oral Indigenous Microbiota in Elderly Adults
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DOI：
10.1128/aem.00651-11
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发表时间：
2011-10-01
影响因子：
4.4
通讯作者：
Yamashita, Yoshihisa
中科院分区：
文献类型：
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作者：
Takeshita, Toru;Yasui, Masaki;Yamashita, Yoshihisa
对于进食困难的老年人，肠内管喂养被广泛用于维持营养，但长期使用会改变口腔生态系统的环境。这项研究通过分析16S rRNA基因来表征管饲老年人的舌部微生物区系。对44例管饲者和54例口饲者(平均年龄86.4+/-6.9岁)的T-RFLP进行了比较。还从每组受试者(15名管饲受试者和16名口服受试者)中获得了一部分受试者的条码焦磷酸测序数据。T-RFLP图谱显示，管饲受试者的微生物区系与口服受试者不同(置换多变量方差分析[perMANOVA]，P<；0.001)。焦磷酸测序数据显示，棒状杆菌、消化链球菌和梭杆菌等22个细菌属在管饲受试者中明显占优势，而在口服受试者中，优势菌属如链球菌和韦龙氏菌的比例要低得多。在正常口腔微生物区系中很少发现的条件致病菌，如纹状棒状杆菌和无乳链球菌，在管饲受试者中经常被发现。肠道喂养的使用扰乱了口腔本土微生物区系，使威胁健康的细菌得以蓬勃发展。
Enteral tube feeding is widely used to maintain nutrition for elderly adults with eating difficulties, but its long-term use alters the environment of the oral ecosystem. This study characterized the tongue microbiota of tube-fed elderly adults by analyzing the 16S rRNA gene. The terminal restriction fragment length polymorphism (T-RFLP) profiles of 44 tube-fed subjects were compared with those of 54 subjects fed orally (average age, 86.4 +/- 6.9 years). Bar-coded pyrosequencing data were also obtained for a subset of the subjects from each group (15 tube-fed subjects and 16 subjects fed orally). The T-RFLP profiles demonstrated that the microbiota of the tube-fed subjects was distinct from that of the subjects fed orally (permutational multivariate analysis of variance [perMANOVA], P < 0.001). The pyrosequencing data revealed that 22 bacterial genera, including Corynebacterium, Peptostreptococcus, and Fusobacterium, were significantly more predominant in tube-fed subjects, whereas the dominant genera in the subjects fed orally, such as Streptococcus and Veillonella, were present in much lower proportions. Opportunistic pathogens rarely detected in the normal oral microbiota, such as Corynebacterium striatum and Streptococcus agalactiae, were often found in high proportions in tube-fed subjects. The oral indigenous microbiota is disrupted by the use of enteral feeding, allowing health-threatening bacteria to thrive.