Fluorescence properties of some farm wastes: implications for water quality monitoring
Fluorescence properties of some farm wastes: implications for water quality monitoring
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DOI：
10.1016/s0043-1354(01)00210-x
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发表时间：
2002-01-01
期刊：
影响因子：
12.8
通讯作者：
Baker, A
中科院分区：
文献类型：
--
作者：
Baker, A
利用荧光激发矩阵（EEM）光谱法分析了一些农业废弃物的荧光特性。调查的农场废弃物是青贮液、猪和牛泥浆以及羊舍废弃物。所有农场废物都表现出高强度的荧光，这可以归因于蛋白质色氨酸。青贮液的特征在于非常高的荧光强度和> 20的初始色氨酸：富里酸样荧光强度比。牛和猪的粪便中色氨酸与富里酸的荧光强度比较低（接近2 -5），色氨酸荧光强度也较低，同时还观察到酪氨酸荧光。羊舍废弃物中色氨酸与富里酸类荧光强度比最低（约0.5 -4.0）。在取样后的50天内，在受控温度条件下重新分析农场废物样品，以研究其荧光特性的稳定性。对于青贮液，色氨酸：观察到富里酸样荧光强度比随时间降低，并且与色氨酸荧光强度的降低有关，提示梭菌分解蛋白质。对于浆料样品，色氨酸：富里酸样荧光强度表现出更可变的时间演变，并且色氨酸荧光强度随着时间的推移而增加;更复杂的荧光信号是由于浆料的相对不均匀性造成的。羊舍废弃物样品表现出更稳定的色氨酸：富里酸样荧光强度比和色氨酸强度，这表明这些样品更稳定，由于其更大的年龄和分解。色氨酸的比例：从调查的农场废物中观察到的富里酸样荧光强度明显高于在大多数河流沃茨中观察到的荧光强度，这表明农场废物污染事件可能在河流沃茨中留下特征，因为它们具有明显的高蛋白质荧光强度。(C)2001爱思唯尔科技有限公司版权所有。
Some farm wastes have been analysed for their fluorescence properties using fluorescence excitation-matrix (EEM) spectroscopy. Farm wastes investigated were silage liquor, pig and cattle slurry, and sheep barn waste. All farm wastes exhibited high intensities of fluorescence that can be attributed to the protein tryptophan. Silage liquor was characterised by a very high fluorescence intensity and an initial tryptophan: fulvic-like fluorescence intensity ratio of > 20. Cattle and pig slurries exhibited a lower tryptophan: fulvic-like fluorescence intensity ratio (similar to2-5) and lower tryptophan fluorescence intensity, and tyrosine fluorescence was also observed. Sheep barn wastes had the lowest tryptophan: fulvic-like fluorescence intensity ratios (similar to0.5-4.0). Farm waste samples were reanalysed under controlled temperature conditions over a period of 50 days after sampling, to investigate the stability of their fluorescence properties. For silage liquor, tryptophan: fulvic-like fluorescence intensity ratios were observed to decrease with time, and were associated with a decrease in tryptophan fluorescence intensity, suggestive of clostridia breakdown of protein. For slurry samples, tryptophan: fulvic-like fluorescence intensity exhibited a more variable time-evolution, and tryptophan fluorescence intensity increased through time; the more complex fluorescence signal is due to the relatively heterogeneous nature of the slurry. Sheep barn waste samples exhibited more stable tryptophan: fulvic-like fluorescence intensity ratio and tryptophan intensities, suggesting these samples were more stable due to their greater age and decomposition. The ratios of tryptophan: fulvic-like fluorescence intensity observed from the farm wastes investigated are significantly higher than those observed in the majority of river waters, suggesting that farm waste pollution events could leave a signature in river waters due to their distinctively high protein fluorescence intensity. (C) 2001 Elsevier Science Ltd. All rights reserved.