PERILIPIN-Dependent Control of Lipid Droplet Structure and Fat Storage in Drosophila
PERILIPIN-Dependent Control of Lipid Droplet Structure and Fat Storage in Drosophila
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DOI：
10.1016/j.cmet.2010.10.001
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发表时间：
2010-11-03
期刊：
影响因子：
29
通讯作者：
Kuehnlein, Ronald P.
中科院分区：
文献类型：
--
作者：
Beller, Mathias;Bulankina, Anna V.;Kuehnlein, Ronald P.
脂滴是富含于脂肪组织中的细胞内细胞器，其控制动物的体脂肪储存。在哺乳动物中，进化上保守的周磷脂蛋白家族成员与脂滴表面相关并参与脂质稳态。在这里，我们表明，果蝇突变体缺乏周脂蛋白PLIN 1是hyperphagic和患有成人发病肥胖症。PLIN 1是脂肪代谢的中心和两面组成部分。它为储存脂质分解提供屏障功能，并通过调节蛋白质进入脂滴表面而作为刺激脂解的关键因素。它还形成脂滴结构，将单室脂肪细胞转化为多室脂肪细胞。我们产生了缺乏所有周脂蛋白家族成员的果蝇，并表明它们表现出受损但功能性的体脂调节。我们的数据揭示了一个基础的和可能古老的脂质稳态系统的存在。
Lipid droplets are intracellular organelles enriched in adipose tissue that govern the body fat stores of animals. In mammals, members of the evolutionarily conserved PERILIPIN protein family are associated with the lipid droplet surface and participate in lipid homeostasis. Here, we show that Drosophila mutants lacking the PERILIPIN PLIN1 are hyperphagic and suffer from adult-onset obesity. PLIN1 is a central and Janus-faced component of fat metabolism. It provides barrier function to storage lipid breakdown and acts as a key factor of stimulated lipolysis by modulating the access of proteins to the lipid droplet surface. It also shapes lipid droplet structure, transforming unilocular into multilocular fat cells. We generated flies devoid of all PERILIPIN family members and show that they exhibit impaired yet functional body fat regulation. Our data reveal the existence of a basal and possibly ancient lipid homeostasis system.