Regional distribution and postnatal changes of D-amino acids in rat brain
Regional distribution and postnatal changes of D-amino acids in rat brain
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DOI：
10.1016/s0304-4165(96)00095-5
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发表时间：
1997-03-15
影响因子：
3
通讯作者：
Imai, K
中科院分区：
文献类型：
--
作者：
Hamase, K;Homma, H;Imai, K
通过先前开发的改进的高灵敏度分析方法研究了大鼠脑中D-氨基酸的区域分布。该方法包括对每种氨基酸进行荧光衍生化，通过反相 HPLC 分离每种氨基酸，然后使用 Pirkle 型手性固定相进行对映体分离。测定大脑、小脑、海马、延髓、垂体和松果体中的D-氨基酸含量。在松果体（3524+/-263 nmol/g，数据为6周龄雄性大鼠）和垂体（80.5+/-9.0 nmol/g）中观察到D-天冬氨酸。除小脑和延髓外，D-丝氨酸存在于大脑的各个区域。仅在垂体中观察到 D-丙氨酸 (25.9+/-4.4 nmol/g)，而在松果体 (3.4+/-0.4 nmol/g) 和海马 (1.6+/-0.07 nmol/g) 中发现 D-亮氨酸。大脑中没有检测到其他 D-氨基酸。雌性大鼠脑垂体中D-天冬氨酸和松果体中D-丝氨酸的含量较高。相比之下，男性垂体中的D-丙氨酸以及松果体和海马中的D-亮氨酸含量较高。还研究了松果体中 D-天冬氨酸和 D-亮氨酸以及垂体中 D-丙氨酸的出生后变化。本文描述的结果表明，大鼠大脑相应区域中的各个 D-氨基酸存在不同的调节机制。
Regional distribution of D-amino acids in rat brain was studied by the modified highly sensitive analytical method which was previously developed. The method includes fluorogenic derivatization of each amino acid, isolation of each amino acid by reverse-phase HPLC, followed by enantiomeric separation with Pirkle-type chiral stationary phases. D-Amino acid contents were determined in the cerebrum, cerebellum, hippocampus, medulla oblongata, pituitary gland and pineal gland. D-Aspartic acid was observed in the pineal gland (3524+/-263 nmol/g, data are for male rats of 6 weeks of age) and the pituitary gland (80.5+/-9.0 nmol/g). D-Serine was found in various regions of the brain except for the cerebellum and medulla oblongata. D-Alanine was observed exclusively in the pituitary gland (25.9+/-4.4 nmol/g), whereas D-leucine was found in the pineal gland (3.4+/-0.4 nmol/g) and the hippocampus (1.6+/-0.07 nmol/g). No other D-amino acids were detected in the brain. The contents of D-aspartic acid in the pituitary gland and D-serine in the pineal gland were higher in female rats. In contrast the contents of D-alanine in the pituitary gland and D-leucine in the pineal gland and the hippocampus were higher in males. Postnatal changes of D-aspartic acid and D-leucine in the pineal gland and D-alanine in the pituitary gland were also investigated. The results described in this paper suggested that distinct regulatory mechanisms exist for individual D-amino acids in the corresponding region of rat brain.