Rapid generation of collagen-based microtissues to study cell-matrix interactions
Rapid generation of collagen-based microtissues to study cell-matrix interactions
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DOI：
10.1142/s2339547816400094
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发表时间：
2016-06-01
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影响因子：
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通讯作者：
Wood, David K.
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文献类型：
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作者：
Brett, Marie-Elena;Crampton, Alexandra L.;Wood, David K.
本研究的目的是创建一种方法来研究生理相关的基于蛋白质的3D组织结构中的细胞-基质相互作用，这种组织结构可以按比例进行大规模筛选，在群体基础上研究细胞-基质相互作用，或者由细胞重塑以构建更大的组织。我们已经开发了一种易于使用的方法来小型化基于蛋白质的组织结构，在体外维持3D环境，同时减轻了与较大的无血管组织结构相关的几个障碍。在这项研究中，我们证明了(i)细胞在被包裹或仅与微组织表面相互作用时都可以与3D环境相互作用，（ii）被包裹的细胞具有很高的存活率，并且，这是第一次，（iii）这种大小尺度（类似于200 μ m）的微组织可以用来量化细胞的收缩性。这个多功能平台应该促进在药物开发和高通量机制生物学的3D体外培养条件下的大规模筛选。
The objective of this study was to create a method for studying cell-matrix interactions in a physiologically relevant 3D protein-based tissue construct that could be scaled up to perform large-scale screens, study cell-matrix interactions on a population basis, or be remodeled by cells to build larger tissues. We have developed an easy-to-use method to miniaturize protein-based tissue constructs that maintains the 3D in vitro environment, while alleviating several obstacles associated with larger avascular tissue constructs. In this study, we demonstrate that ( i) cells can interact with the 3D environment both while encapsulated or while interacting only with the surface of the microtissues, ( ii) encapsulated cells are highly viable and, for the first time, ( iii) microtissues on this size scale (similar to 200 mu m) can be used to quantify cell contractility. This versatile platform should facilitate large-scale screens in 3D in vitro culture conditions for drug development and high throughput mechanistic biology.