Structural Basis of Karrikin and Non-natural Strigolactone Perception in Physcomitrella patens
Structural Basis of Karrikin and Non-natural Strigolactone Perception in Physcomitrella patens
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DOI：
10.1016/j.celrep.2019.01.003
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发表时间：
2019-01-22
期刊：
影响因子：
8.8
通讯作者：
Chory, Joanne
中科院分区：
文献类型：
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作者：
Burger, Marco;Mashiguchi, Kiyoshi;Chory, Joanne
在植物中，STRG内酯是通过双重受体-水解酶DWARF14(D14)识别的。D14属于α/β水解酶超家族，在结构上与Karrikin受体Karrikin不敏感2(KAI2)相似。由于它包含11个高度相关但配体特异性未知的家族成员，因此是研究该受体家族的理想模型系统。我们介绍了三个Physcomitrella D14/KAI2类蛋白的晶体结构，并描述了导致疏水底物峡谷永久加宽的环机制。我们已经确定了以高度立体选择性的方式特异性地识别karrikin KAR(1)和非天然的三叉内酯异构体(类似于)-5-脱氧去氧雌酚的蛋白质分支。
In plants, strigolactones are perceived by the dual receptor-hydrolase DWARF14 (D14). D14 belongs to the superfamily of alpha/beta hydrolases and is structurally similar to the karrikin receptor KARRIKIN INSENSITIVE 2 (KAI2). The moss Physcomitrella patens is an ideal model system for studying this receptor family, because it includes 11 highly related family members with unknown ligand specificity. We present the crystal structures of three Physcomitrella D14/KAI2-like proteins and describe a loop-based mechanism that leads to a permanent widening of the hydrophobic substrate gorge. We have identified protein clades that specifically perceive the karrikin KAR(1) and the non-natural strigolactone isomer (similar to)-5-deoxystrigol in a highly stereoselective manner.