Up regulation of annexin A2 on murine H22 hepatocarcinoma cells induced by cartilage polysaccharide
Up regulation of annexin A2 on murine H22 hepatocarcinoma cells induced by cartilage polysaccharide
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DOI：
10.1016/j.canep.2010.10.004
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发表时间：
2011-10-01
影响因子：
2.6
通讯作者：
Zhang, Hong
中科院分区：
文献类型：
--
作者：
Liu, Anjun;Liu, Dongyue;Zhang, Hong
本实验研究了软骨多糖诱导的H22肝癌细胞制备的肿瘤疫苗的抗肿瘤作用。我们发现有特异性抗原与免疫小鼠血清中的抗原特异性抗体结合。western blot分析结果显示，约36 kDa使特异性抗体在免疫小鼠的抗血清中出现特异性抗体，而在诱导48 h时出现最佳免疫效果。二维电泳分析发现特异性抗原为膜联蛋白A2，这是一种糖基化蛋白，含有糖基化位点，与肿瘤的发生、发展、侵袭和转移密切相关。蛋白质组学表明，软骨多糖诱导后，膜联蛋白A2的数量和构象都发生了变化。最后，我们发现软骨多糖诱导的H22细胞膜联蛋白A2 mRNA显著升高。综上所述，我们的数据表明，膜联蛋白A2是一种特异性抗原，在抗肿瘤免疫应答和激活免疫系统中发挥了关键作用。为肿瘤免疫预防提供了新的思路，是一种有潜力的肿瘤疫苗。(C) 2010 Elsevier Ltd.版权所有。
In this study, we investigated the antitumor effect of a tumor vaccine prepared from H22 hepatocarcinoma cells induced by cartilage polysaccharide. We found out there were specific antigens which combined with antigen-specific antibodies from immune murine serum. Results of western blot analysis showed that about 36 kDa make specific antibodies appeared specific antibodies in antiserum of immune mice, whereas the best immune effects became visible at the induction time of 48 h. Analyses of 2-dimensional electrophoresis identified the specific antigen was annexin A2, which was a glycosylated protein that contained a glycosylation site, closely related to oncogenesis, cancer development, invasion and metastasis. Proteomics indicated that both quantity and conformation of annexin A2 were changed after induced by cartilage polysaccharide. Lastly, we found there was a major increase of annexin A2 mRNA on H22 cells induced by cartilage polysaccharide. In summary, our data suggested that annexin A2, a specific antigen played a key role in antitumor immune response and activating the immune system. It would be a potential type of tumor vaccine which provided new ideas for tumor immunoprophylaxis. (C) 2010 Elsevier Ltd. All rights reserved.