Iron-dependent transcription of the frpB gene of Helicobacter pylori is controlled by the fur repressor protein
Iron-dependent transcription of the frpB gene of Helicobacter pylori is controlled by the fur repressor protein
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DOI：
10.1128/jb.183.16.4932-4937.2001
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发表时间：
2001-08-01
影响因子：
3.2
通讯作者：
Scarlato, V
中科院分区：
文献类型：
--
作者：
Delany, I;Pacheco, ABF;Scarlato, V
我们已经高效表达和纯化了幽门螺杆菌毛皮蛋白，并分析了它与铁摄取(frpB和ceuE)和氧自由基解毒(kata和tsaA)相关差异基因的基因间隔区的相互作用。DNase I足迹分析表明，Fur特异性地结合到与P-frpB启动子重叠的高亲和力部位，以及位于frpB-Kata和ceuE-tsaA基因间隔区启动子上游的低亲和力部位。构建了一个等基因的Fur突变体，表明Fur调节P-frpB启动子对铁的转录反应。相比之下，无论是皮草还是铁对其他促进剂都没有影响。
We have overexpressed and purified the Helicobacter pylori Fur protein and analyzed its interaction with the intergenic regions of divergent genes involved in iron uptake (frpB and ceuE) and oxygen radical detoxification (katA and tsaA). DNase I footprint analysis showed that Fur binds specifically to a high-affinity site overlapping the P-frpB promoter and to low-affinity sites located upstream from promoters within both the frpB-katA and ceuE-tsaA intergenic regions. Construction of an isogenic fur mutant indicated that Fur regulates transcription from the P-frpB promoter in response to iron. In contrast, no effect by either Fur or iron was observed for the other promoters.